نبذة مختصرة : Objective. To determine the circulation of West Nile virus (WNV) in horses from the eastern Yucatán, Mexico. Materials and Methods. For convenience, 184 horses from 23 production units in the municipalities of Tizimín and Panabá, Yucatán, were studied. A blood serum sample was obtained from each studied horse and evaluated with an immunoglobulin M antibody-capture enzyme-linked immunosorbent assay (MAC-ELISA) to detect immunoglobulin M (IgM) against WNV. Additionally, positive reactor and suspect serum samples were analyzed by reverse transcription polymerase chain reaction (RT-PCR) to detect viral RNA. Results. Eight studied horses were seropositive (4.3%, 8/184) to WNV and two were suspects (1.1%, 2/184). All serum samples were negative by RT-PCR. Conclusions. The detection of IgM specific against WNV in the studied horses shows recent infections with the virus and indicates its circulation in eastern Yucatán, Mexico. ; Objetivo. Determinar la circulación del virus del Oeste del Nilo (VON) en caballos del oriente de Yucatán, México. Materiales y métodos. Por conveniencia se estudiaron 184 caballos localizados en 23 unidades de producción de los municipios de Tizimín y Panabá, Yucatán. De cada caballo se obtuvo una muestra de suero sanguíneo, la cual fue evaluada con un ensayo de inmunoabsorción enzimática de captura (MAC-ELISA) para detectar inmunoglobulinas de clase M (IgM) específicas contra VON. Adicionalmente, los sueros seroreactores y los sospechosos fueron analizados con una reacción en cadena de la polimerasa por retrotranscripción (RT-PCR) para detectar ARN viral. Resultados. Ocho caballos estudiados fueron seroreactores en la ELISA-MAC (4.3%, 8/184) y dos fueron sospechosos (1.1%, 2/184). Todos estos sueros fueron negativos a la RT-PCR. Conclusiones. La detección de IgM específicas contra VON en los caballos estudiados, demuestra infecciones recientes e indica la circulación del virus en el oriente de Yucatán.
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