Serological, cultural and molecular evidence of Brucella melitensis infection in goats in Al Jabal Al Akhdar, Sultanate of Oman

Brucellosis, one of the most common zoonotic diseases and has significant public health and economic importance worldwide. Few studies and reports have been performed to estimate the true prevalence of animal brucellosis in the Sultanate of Oman; however, no incidence of the disease was previously reported in Al Jabal Al Akhdar. The purpose of this study was to investigate the prevalence of brucellosis in goats in eight villages in Al Jebal Al Akhdar, Sultanate of Oman, namely: Al Aqaieb, Al Helailat, Al Ghilayil, Hail Al Hedap, Da'an Al Hamra, Shnoot, Al Qasha'e and Al Sarah , Al Jabal Al Akhdar in the Sultanate of Oman. In this study we used different diagnostic serological tests, namely, RBT , I‐ ELISA and CFT to study the prevalence of Brucella infection in goats in Al Jabal Al Akhdar. Statistical analysis using Kappa statistics was used to compare the performance of the serological tests. Biochemical tests and species‐specific Multiplex PCR were used to identify the brucella species involved in the infection. A structured questionnaire and Chi‐square (x 2 ) statistical analysis was used to identify related brucellosis risk factors. This study is the first to reveal brucellosis infection in goats in eight villages in Al Jebal Al Akhdar, Sultanate of Oman, namely: Al Aqaieb, Al Helailat, Al Ghilayil, Hail Al Hedap, Da'an Al Hamra, Shnoot, Al Qasha'e and Al Sarah , with an overall seroprevalence of 11.1%. The study also compared the performance of three different serological tests, namely, RBT , I‐ ELISA and CFT . Statistical analysis using Kappa statistics showed that the degree of agreement was best seen between RBT and CFT (96%), followed by RBT , I‐ ELISA (91.4%) and CFT and I‐ ELISA (89.2%). Biochemical tests and species‐specific Multiplex PCR showed the typical profile for B. melitensis . A structured questionnaire and Chi‐square (x 2 ) statistical analysis indicated that the presence of abortion is the major risk factor for the prevalence of brucellosis, whereas age and sex were not significant ...