Metallic micronutrients are associated with the structure and function of the soil microbiome

The relationship between metallic micronutrients and soil microorganisms, and thereby soil functioning, has been little explored. Here, we investigate the relationship between metallic micronutrients (Fe, Mn, Cu, Zn, Mo and Ni) and the abundance, diversity and function of soil microbiomes. In a survey across 180 sites in China, covering a wide range of soil conditions the structure and function of the soil microbiome are highly correlated with metallic micronutrients, especially Fe, followed by Mn, Cu and Zn. These results are robust to controlling for soil pH, which is often reported as the most important predictor of the soil microbiome. An incubation experiment with Fe and Zn additions for five different soil types also shows that increased micronutrient concentration affects microbial community composition and functional genes. In addition, structural equation models indicate that micronutrients positively contribute to the ecosystem productivity, both directly (micronutrient availability to plants) and, to a lesser extent, indirectly (via affecting the microbiome). Our findings highlight the importance of micronutrients in explaining soil microbiome structure and ecosystem functioning ; This study was supported by the National Science Foundation of China (41991334 to J.X. and Z.D., 41721001 to J.X.), the National Key Research and Development Program of China (2019YFC1803704 to Z.D.) and the Modern Agricultural Industry Technology System of China (CARS-01 to J.X.). M.D-B. acknowledges support from TED2021-130908B-C41/AEI/10.13039/501100011033/Unión Europea NextGenerationEU/PRTR andfrom the Spanish Ministry of Science and Innovation for the I + D + iproject PID2020-115813RA-I00 funded by MCIN AEI/10.13039/501100011033