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Methanohalophilus profundi sp. nov., a methylotrophic halophilic piezophilic methanogen isolated from a deep hypersaline anoxic basin

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اقرأ أكثر
  • معلومة اضافية
    • Contributors:
      Laboratoire de Microbiologie des Environnements Extrêmophiles (LM2E); Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS); ABiMS - Informatique et bioinformatique = Analysis and Bioinformatics for Marine Science (ABIMS); Fédération de recherche de Roscoff (FR2424); Station biologique de Roscoff = Roscoff Marine Station (SBR); Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR); Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS); Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS); Insitute for Coastal Marine Environment; National Research Council of Italy; This study was funded by the European Union program MaCuMBA (grant agreement 311975) and the MAMBA project (FP7-KBBE-2008–226977). Research was supported by UBO, CNRS and Ifremer.; ANR-11-INBS-0013,IFB (ex Renabi-IFB),Institut français de bioinformatique(2011); European Project: 311975,EC:FP7:KBBE,FP7-KBBE-2012-6-singlestage,MACUMBA(2012); European Project: 226977,EC:FP7:KBBE,FP7-KBBE-2008-2B,MAMBA(2009)
    • بيانات النشر:
      CCSD
      Elsevier
    • الموضوع:
      2020
    • نبذة مختصرة :
      International audience ; A novel anaerobic methylotrophic halophilic methanogen strain SLHTYRO T was isolated from a deep hypersaline anoxic basin called "Tyro" located in the Eastern Mediterranean Sea. Cells of SLHTYRO T were motile cocci. The strain SLHTYRO T grew between 12 and 37 °C (optimum 30 °C), at pH between 6.5 and 8.2 (optimum pH 7.5) and salinity from 45 to 240 g L −1 NaCl (optimum 135 g L −1). Strain SLHTYRO T was methylotrophic methanogen able to use methylated compounds (trimethylamine, dimethylamine, monomethylamine and methanol). Strain SLHTYRO T was able to grow at in situ hydrostatic pressure and temperature conditions (35 MPa, 14 °C). Phylogenetic analysis based on 16S rRNA gene and mcrA gene sequences indicated that strain SLHTYRO T was affiliated to genus Methanohalophilus within the order Methanosarcinales. It shared >99.16% of the 16S rRNA gene sequence similarity with strains of other Methanohalophilus species. Based on ANIb, AAI and dDDH measurements, and the physiological properties of the novel isolate, we propose that strain SLHTYRO T should be classified as a representative of a novel species, for which the name Methanohalophilus profundi sp. nov. is proposed; the type strain is SLHTYRO T (=DSM 108854 = JCM 32768 = UBOCC-M-3308).
    • Relation:
      info:eu-repo/semantics/altIdentifier/pmid/32847782; info:eu-repo/grantAgreement/EC/FP7/311975/EU/Marine Microorganisms: Cultivation Methods for Improving their Biotechnological Applications/MACUMBA; info:eu-repo/grantAgreement/EC/FP7/226977/EU/Marine Metagenomics for New Biotechnological Applications/MAMBA; PUBMED: 32847782
    • الرقم المعرف:
      10.1016/j.syapm.2020.126107
    • الدخول الالكتروني :
      https://pasteur.hal.science/pasteur-03260922
      https://pasteur.hal.science/pasteur-03260922v1/document
      https://pasteur.hal.science/pasteur-03260922v1/file/S072320202030062X.pdf
      https://doi.org/10.1016/j.syapm.2020.126107
    • Rights:
      http://creativecommons.org/licenses/by-nc/ ; info:eu-repo/semantics/OpenAccess
    • الرقم المعرف:
      edsbas.9B48FDFF