Additional file 1 of Media optimization for SHuffle T7 Escherichia coli expressing SUMO-Lispro proinsulin by response surface methodology

Additional file 1: Figure S1. Original Figure 8A. POI soluble expression and Purification. Coomassie stained 12% SDS-PAGE: POI soluble expression in LB and OM-I media. M. Protein Marker. 1–3: POI soluble expression in LB media. 4–6: POI soluble expression in OM-I media. Figure S2. Original Figure 8B. POI soluble expression and Purification. Coomassie stained 12% SDS-PAGE: SU-INS POI IMAC purification. M. Protein Ladder.1: Cell lysate supernatant (Unpurified), 2: Purified POI. Figure S2. Original Figure 9B. SU-INS POI soluble expression in fermentor. Coomassie stained 12% SDS-PAGE: M. Protein Ladder.1: Post-induction cell lysate supernatants