A Toxicity Study on Sangu Parpam

The present study was conducted to know single dose toxicity of SANGU PARPAM on female Wistar Albino Rats. AIM AND OBJECTIVES: AIM: The aim of this study is to evaluate the toxicity study on “SANGU PARPAM”. OBJECTIVES: 1. To collect and purify the raw materials based on literature evidence. 2. To perform compound analysis for the purified raw drug samples. 3. To prepare the medicine based on siddha literature evidence. 4. To estimate the presence of chemical constituents by performing elemental analysis. 5. To analyse its safety, the medicine is subjected for acute oral toxicity evaluation on rodents when the drug is given in single dose through oral administration at various dose levels. 6. To analyse its safety, the medicine is subjected for repeated oral dose (subacute) toxicity study evaluation on rodents when the drug is given for 28 days. The study was conducted using 15 female Wistar Albino Rats. The female animals were selected for study of 8-12 weeks old with weight range of within ±20% of mean body weight at the time of randomization. The groups were numbered as group I, II, III, IV and V and dose with 5mg/kg, 50mg/kg, 300mg/kg and 2000mg/kg of SANGU PARPAM. The drug was administered by oral route single time and observed for 14 days. Daily the animals were observed for clinical signs and mortality. Body weight of animals was recorded once in a week. There were no physical and general behavioral changes observed in Wistar Albino Rats of 5mg/kg, 50mg/kg, 300mg/kg and 2000mg/kg to rats during 14 days. Body weight of all animals did not reveal any significant change as compared to vehicle control group. Food consumption of all group animals was normal. Mortality was not observed in all treatment groups. CONCLUSION: The study shows that SANGU PARPAM did not produce any toxic effect and mortality at dose of 5mg/kg, 50mg/kg, 300mg/kg and 2000mg/kg to rats. So NO Observed-Adverse-Effect-Level(NOAEL) of SANGU PARPAM is 2000mg/kg.