The Effect of hyperthermic chemotherapy treatment to human gastric cancer cells

Gastric cancer is a form of cancer, which requires many deaths in Lithuania and around the world. Every year in Lithuania this desease kills about 700 patients. New therapies are being investigated in order to help patients get well or even slightly increase their life expectancy. Hyperthermic intraperitoneal chemotherapy (HIPEC) is a method of treatment, which is quite recently applied in Lithuania. Most of the science publications (total found 700) are about clinical effectiveness of HIPEC, but there is a lack of fundamental knowledge about the effect of different types of cancer cells, including gastric cancer. The aim of the study was to determine hyperthermic chemotherapy effect on human gastric cancer cells. The effect of hyperthermic chemotherapy was investigated using MTT cell viability determination method, flow cytometry for apoptosis measurement and analysis of cells morphology using microscopy. The results demonstrated that the effect of temperature 41°C - 45°C, AGS cell viability decreased by approximately 32%, compared to the control cells (AGS cells affected by in vitro incubation at 37°C). The maximum effect of chemotherapeutic drug cisplatin to decrease AGS cells vitality occurred, when drug concentration was increased to 200 uM. The combinational effect of hyperthermia and chemotherapy to maximally decrease viability of AGS cells was observed at 45°C temperature and cysplatin concentration of 200 uM, compared with the control group.