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The Voltage-Gated Sodium Channel Na(v)1.8 Is Expressed in Human Sperm

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اقرأ أكثر حفظ في قائمتي
  • معلومة اضافية
    • بيانات النشر:
      Public Library of Science
    • الموضوع:
      2013
    • Collection:
      ADDI: Repositorio Institucional de la Universidad del País Vasco / Euskal Herriko Unibertsitatea (UPV/EHU - Basque Country University)
    • نبذة مختصرة :
      The role of Na+ fluxes through voltage-gated sodium channels in the regulation of sperm cell function remains poorly understood. Previously, we reported that several genes encoding voltage-gated Na+ channels were expressed in human testis and mature spermatozoa. In this study, we analyzed the presence and function of the TTX-resistant VGSC a subunit Na(v)1.8 in human capacitated sperm cells. Using an RT-PCR assay, we found that the mRNA of the gene SCN10A, that encode Na-v1.8, was abundantly and specifically expressed in human testis and ejaculated spermatozoa. The Na-v1.8 protein was detected in capacitated sperm cells using three different specific antibodies against this channel. Positive immunoreactivity was mainly located in the neck and the principal piece of the flagellum. The presence of Na-v1.8 in sperm cells was confirmed by Western blot. Functional studies demonstrated that the increases in progressive motility produced by veratridine, a voltage-gated sodium channel activator, were reduced in sperm cells preincubated with TTX (10 mu M), the Na-v1.8 antagonist A-803467, or a specific Na-v1.8 antibody. Veratridine elicited similar percentage increases in progressive motility in sperm cells maintained in Ca2+-containing or Ca2+-free solution and did not induce hyperactivation or the acrosome reaction. Veratridine caused a rise in sperm intracellular Na+, [Na+](i), and the sustained phase of the response was inhibited in the presence of A-803467. These results verify that the Na+ channel Na-v1.8 is present in human sperm cells and demonstrate that this channel participates in the regulation of sperm function. ; This work was financially supported by grants from Junta de Andalucia (P08-CVI-04185) and Ministerio de Economia y Competitividad (CTQ2011-25564), Spain, with joint financing by FEDER and FSE funds from the European Union. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
    • File Description:
      application/pdf
    • ISSN:
      1932-6203
    • Relation:
      info:eu-repo/grantAgreement/MINECO/CTQ2011-25564; http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0076084; PLoS ONE 8(9) : (2013) // e76084; http://hdl.handle.net/10810/11261
    • الرقم المعرف:
      10.1371/journal.pone.0076084
    • Rights:
      info:eu-repo/semantics/openAccess ; © 2013 Cejudo-Román et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
    • الرقم المعرف:
      edsbas.974FEBED