Dysregulation of Iron Metabolism-Linked Genes at Myocardial Tissue and Cell Levels in Dilated Cardiomyopathy

settings Order Article Reprints Open AccessArticle Dysregulation of Iron Metabolism-Linked Genes at Myocardial Tissue and Cell Levels in Dilated Cardiomyopathy by Ilaria Massaiu 1,†, Jeness Campodonico 1,†, Massimo Mapelli 1 [ORCID] , Elisabetta Salvioni 1, Vincenza Valerio 1, Donato Moschetta 1,2 [ORCID] , Veronika A. Myasoedova 1 [ORCID] , Maria Domenica Cappellini 3,4 [ORCID] , Giulio Pompilio 1,5, Paolo Poggio 1,*,‡ [ORCID] and Piergiuseppe Agostoni 1,4,*,‡ 1 Centro Cardiologico Monzino, IRCCS, 20138 Milan, Italy 2 Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, 20122 Milan, Italy 3 UOC General Medicine, Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy 4 Department of Clinical Sciences and Community Health, University of Milan, 20122 Milan, Italy 5 Department of Biomedical, Surgical and Dental Sciences, University of Milan, 20122 Milan, Italy * Authors to whom correspondence should be addressed. † These authors equally contributed as first author. ‡ These authors equally contributed as last author. Int. J. Mol. Sci. 2023, 24(3), 2887; https://doi.org/10.3390/ijms24032887 Received: 23 December 2022 / Revised: 19 January 2023 / Accepted: 26 January 2023 / Published: 2 February 2023 (This article belongs to the Special Issue Iron Metabolism in Health and Disease) Download Browse Figures Versions Notes Abstract In heart failure, the biological and clinical connection between abnormal iron homeostasis, myocardial function, and prognosis is known; however, the expression profiles of iron-linked genes both at myocardial tissue and single-cell level are not well defined. Through publicly available bulk and single-nucleus RNA sequencing (RNA-seq) datasets of left ventricle samples from adult non-failed (NF) and dilated cardiomyopathy (DCM) subjects, we aim to evaluate the altered iron metabolism in a diseased condition, at the whole cardiac tissue and single-cell level. From the bulk RNA-seq data, we found 223 iron-linked genes expressed at the ...