Contributors: Instituto de Investigação e Inovação em Saúde (I3S); Universidade do Porto = University of Porto; Instituto de Biologia Molecular e Celular (IBMC); Faculdade de Farmácia da Universidade do Porto (FFUP); Infection et Immunité paludéennes - Malaria Infection and Immunity; Institut Pasteur Paris (IP); This work was supported by funds from the Fundação para a Ciência e Tecnologia (FCT)/Ministério da Educação e Ciência (MEC) co-funded by FEDER (EXPL/JTAVARES-IF/00881/2012/CP0158/CT0005, EXPL/IMI-MIC/1331/2013) under the Partnership agreement PT2020, through the Research Unit No.4293, the Institut Paris – Paris, the French National Research Agency (Grant No. ANR-10-JCJC-1302-PlasmoPEP), the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence “Integrative Biology of Emerging Infectious Diseases” (Grant No. ANR-10-LABX-62-IBEID). JT is an Investigator FCT funded by National funds through FCT and co-funded through European Social Fund within the Human Potential Operating Programme. DC is funded by FCT (SFRH/BD/123734/2016).; We would like to acknowledge the team of the Center of Production and Infection of Anopheles (CEPIA), Institut Pasteur, in particular Marek Szatanik, Catherine Thouvenot and Sylvain Golba for providing the Anopheles stephensi female mosquitos; the team of the Platform of Dynamic Imaging, Institut Pasteur, in particular Spencer Shorte and Marie-Anne Nicola for the help with bioluminescence imaging; Martin Taylor from the London School of Hygiene and Tropical Medicine for kindly providing the construct used to generate the T. b. brucei Lister 427 strain expressing the red-shifted luciferase.; ANR-10-JCJC-1302,PlasmoPEP,Imagerie in vivo de la phase pre-erythrocitaire du paludisme(2010); ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010)
نبذة مختصرة : International audience ; Hematogenous dissemination followed by tissue tropism is a characteristic of the infectious process of many pathogens including those transmitted by blood-feeding vectors. After entering into the blood circulation, these pathogens must arrest in the target organ before they infect a specific tissue. Here, we describe a non-invasive method to visualize and quantify the homing of pathogens to the host tissues. By using in vivo bioluminescence imaging we quantify the accumulation of luciferase-expressing parasites in the host organs during the first minutes following their intravascular inoculation in mice. Using this technique we show that in the malarial infection, once in the blood circulation, most of bioluminescent Plasmodium berghei sporozoites, the parasite stage transmitted to the host skin by a mosquito bite, rapidly home to the liver where they invade and develop inside hepatocytes. This homing is specific to this developmental stage since blood stage parasites do not accumulate in the liver, as well as extracellular Trypanosoma brucei bloodstream forms and liver-infecting Leishmania infantum amastigotes. Finally, this method can be used to study the dynamics of tissue tropism of parasites, dissect the molecular and cellular basis of their increased arrest in organs and to evaluate immune interventions designed to block this targeted interaction.
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