Would Colloidal Gold Nanocarriers Present An Effective Diagnosis Or Treatment For Ischemic Stroke?

Hamed Amani,1,2,* Ebrahim Mostafavi,3,* Mahmoud Reza Alebouyeh,4,* Hamidreza Arzaghi,5 Abolfazl Akbarzadeh,6 Hamidreza Pazoki-Toroudi,7 Thomas J Webster3 1Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran; 2Physiology Research Center, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran; 3Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 4Anesthesia Department, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran; 5Department of Medical Biotechnology, Faculty of Allied Medical Sciences, Iran University of Medical Sciences, Tehran, Iran; 6Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; 7Physiology Research Center and Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran*These authors contributed equally to this workCorrespondence: Thomas J WebsterDepartment of Chemical Engineering, Northeastern University, Boston, MA, USAEmail th.webster@neu.eduHamidreza Pazoki-ToroudiPhysiology Research Center and Department of Physiology, Iran University of Medical Sciences, P.O. Box 14515-763, Tehran, IranFax +98-21-88675760Email pazoki49@gmail.comIntroduction: This study was conducted to evaluate OX26-PEG-coated gold nanoparticles (GNPs) (OX26@GNPs) as a novel targeted nanoparticulate system on cell survival after ischemic stroke.Materials and methods: Dynamic light scattering (DLS), zeta sizer, and transmission electron microscopy (TEM) were performed to characterize the OX26@GNPs. The effect of OX26@GNPs on infarct volume, neuronal loss, and necroptosis was evaluated 24 h after reperfusion using 2, 3,5-Triphenyltetrazolium chloride (TTC) staining, Nissl staining and Western blot assay, respectively.Results: Conjugation of OX26-PEG to the surface of the 25 nm colloidal gold particles increased their size to 32±2 nm, while a zeta potential change of −40.4 to ...