نبذة مختصرة : Chemokines are believed to play an important role in immunopathogenesis of different joint diseases. One of important problems in studies of synovial fluid is determination of normal level of chemokines from control patients. In our study we determined concentrations of number of chemokines in relatively normal synovial fluid from subjects with background traumatic joint injury without systemic or local inflammation at the moment of study. Results for several CC- and CXC-chemokines are shown for the first time. Therefore, our data can be further used in analysis of synovial fluid chemokine profile in studies of joint disease of different etiology. ; В настоящее время в иммунопатогенезе различных заболеваний суставов большая роль отводится хемокинам. Существенной проблемой при исследовании образцов синовиальной жидкости от больных с патологией суставов является определение контрольного (нормального) уровня хемокинов. В данной работе было проведено определение содержания целой панели хемокинов в относительно нормальной синовиальной жидкости в группе пациентов с травмами суставов в анамнезе и с отсутствием на момент обследования признаков внутри- и внесуставного воспаления. Данные для ряда хемокинов СС- и CXC-классов получены впервые. Эти результаты можно в дальнейшем использовать для изучения воспалительных заболеваний суставов различной этиологии.
Relation: https://www.mimmun.ru/mimmun/article/view/685/688; Zhebrun D.A., Maslyanskiy A.L., Titov A.G., Patrukhin A.P., Kostareva A.A., Gol`tseva I.S, Totolian Areg A. Opredelenie mRNK angiogennykh i angiostaticheskikh khemokinov i ikh retseptorov v sinovial`noy obolochke metodom kolichestvennoy PTSR v real`nom vremeni [Analysis of expression of angiogenic and angiostatic chemokines and their receptors in synovial tissue by quantitative real-time PCR]. Meditsinskaya immunologiya – Medical Immunology, 2013, vol. 15, no. 6, pp. 525-534.; Beekhuizen M., Gierman L.M., Creemers L.B., Dhert W.J., Huizinga T.W, Van Osch G.J., Zuurmond A.M. A descriptive study of synovial fluid changes in cytokine, chemokine and growth factor levels between osteoarthritis patients and healthy donors. Osteoarthritis and Cartilage, 2012, vol. 20, s. 1, pp. S79-S80.; Beekhuizen M., Gierman L.M., van Spil W.E., Van Osch G.J., Huizinga T.W., Saris D.B., Creemers L.B., Zuurmond A.M. An explorative study comparing levels of soluble mediators in control and osteoarthritic synovial fluid. Osteoarthritis and Cartilage, 2013, vol. 21, pp. 918-922.; Blades M.C., Ingegnoli F., Wheller S.K., Manzo A., Wahid S, Panayi G.S., Perretti M., Pitzalis C. Stromal cell-derived factor 1 (CXCL12) induces monocyte migration into human synovium transplanted onto SCID Mice. Arthritis Rheum., 2002, vol. 46, no. 3, pp. 824-836.; De Jager W., Hoppenreijs E.P., Wulffraat N.M., Wedderburn L.R., Kuis W., Prakken B.J. Blood and synovial fluid cytokine signatures in patients with juvenile idiopathic arthritis: a cross-sectional study. Ann. Rheum. Dis., 2007, vol. 66, no. 5, pp. 589-598.; Hampel U., Sesselmann S., Iserovich P., Sel S., Paulsen F., Sack R. Chemokine and cytokine levels in osteoarthritis and rheumatoid arthritis synovial fluid. J. of Immunological Methods, 2013, vol. 396, pp. 134-139.; Hitchon C.A., Alex P., Erdile L.B., Frank M.B., Dozmorov I., Tang Y., Wong K., Centola M., El-Gabalawy H.S. A distinct multicytokine profile is associated with anti-cyclical citrullinated peptide antibodies in patients with early untreated inflammatory arthritis. J. Rheumatol., 2004, vol. 31, no. 12, pp. 2336-2346.; K nig A., Krenn V., Toksoy A., Gerhard N., Gillitzer R. MIG, GROa and RANTES messenger RNA expression in lining layer, infiltrates and different leucocyte populations of synovial tissue from patients with rheumatoid arthritis, psoriatic arthritis and osteoarthritis. Virchows Arch., 2000, vol. 436, pp. 449-458.; Pierer M., Rethage J., Seibl R., Lauener R., Brentano F., Wagner U., Hantzschel H., Michel B.A., Gay R.E., Gay S., Kyburz D. Chemokine secretion of rheumatoid arthritis synovial fibroblasts stimulated by Tolllike receptor 2 ligands. J. Immunol., 2004, vol. 172, no. 2, pp. 1256-1265.; Pierzchala A.W., Kusz D.J., Hajduk G. CXCL8 and CCL5 Expression in Synovial Fluid and Blood Serum in Patients with Osteoarthritis of the Knee. Arch. Immunol. Ther. Exp., 2011, vol. 59, no. 2, pp. 151-155.; Vangsness C.T. Jr., Burke W.S., Narvy S.J., MacPhee R.D., Fedenko A.N. Human knee synovial fluid cytokines correlated with grade of knee osteoarthritis-a pilot study. Bull. NYU Hosp. Jt. Dis., 2011, vol. 69, no. 2, pp. 122-127.; https://www.mimmun.ru/mimmun/article/view/685
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