Molecular cloning of vertebrate growth hormone receptor complementary DNAs.

by Yam Kwok Fai. ; Year shown on spine: 1997. ; Thesis (M.Phil.)--Chinese University of Hong Kong, 1996. ; Includes bibliographical references (leaves 141-149). ; Acknowledgments --- p.i ; List of Contents --- p.ii ; List of Figures --- p.viii ; List of Tables --- p.xii ; List of Primers --- p.xiii ; Abbreviations --- p.xiv ; Chapter Chapter 1 --- Introduction ; Chapter 1.1 --- Growth Hormone (GH) --- p.1 ; Chapter 1.2 --- Growth Hormone Receptor (GHR) --- p.3 ; Chapter 1.2.1 --- Tissue Distribution of GHR --- p.4 ; Chapter 1.2.2 --- Biosynthesis and Degradation of GHR --- p.6 ; Chapter 1.2.3 --- Regulation of GHR Level --- p.7 ; Chapter 1.2.4 --- The Structure of GHR --- p.9 ; Chapter 1.2.5 --- The Structure of GHR Gene --- p.13 ; Chapter 1.2.6 --- Growth Hormone Binding Protein (GHBP) --- p.14 ; Chapter 1.2.7 --- The GH/Prolactin/Cytokine/Erythropoietin Receptor Superfamily --- p.15 ; Chapter 1.2.8 --- Proposed Signal Transduction Pathway --- p.17 ; Chapter 1.2.9 --- GHR Related Dwarfism --- p.22 ; Chapter i). --- Substitution of certain amino acid residues in the extracellular domain --- p.22 ; Chapter ii). --- Deletion of the extracellular domain --- p.23 ; Chapter a). --- deletion of a small portion of the binding protein ; Chapter b). --- deletion of a large portion of the binding protein ; Chapter c). --- deletion of a large portion of the binding domain and the whole transmembrane domain ; Chapter iii). --- Associated with normal GHBP --- p.24 ; Chapter 1.3 --- Objectives of Cloning Vertebrate GHR cDNAs --- p.24 ; Chapter Chapter 2 --- General Experimental Methods ; Chapter 2.1 --- Preparation of Ribonuclease Free Reagents and Apparatus --- p.26 ; Chapter 2.2 --- Isolation of Total RNA --- p.26 ; Chapter 2.3 --- Isolation of mRNA --- p.26 ; Chapter a). --- directly from tissue ; Chapter b). --- from isolated total RNA ; Chapter 2.4 --- Spectrophotometric Quantification and Qualification of DNA and RNA --- p.29 ; Chapter 2.5 --- First Strand cDNA Synthesis --- p.29 ; Chapter 2.6 --- Polymerase Chain ...