Factors affecting pregnancy rate in recipients of embryos produced in vitro under high altitude conditions ; Factores que afectan la tasa de preñez en receptoras de embriones producidos in vitro bajo condiciones de altura

The aim of this study was to determine the factors that affect pregnancy in recipient females of embryos produced in vitro under high altitude conditions. The study was carried out at the Universidad Nacional del Altiplano (UNA), located in Puno, Peru, at an altitude of 3970 m. 50 recipient females (nulliparous, primiparous and multiparous) Brown Swiss were used. Ovaries were collected from cows at a local slaughterhouse and taken to the UNA animal reproduction laboratory, where the oocytes were collected, and in vitro maturation and fertilization, culture and embryo evaluation were carried out. The transport time to the laboratory was 2 to 3 h. Quantitative factors such as body condition score (CC), diameter of the corpus luteum (CL) and number of parities, and qualitative factors such as the place of deposit of the embryo (right or left horn) and embryo quality, were evaluated using the Wilcoxon and Chi test. square, respectively. Likewise, a binary logistic regression was applied to study all the factors together. The highest pregnancy rate occurred when the recipients had a 2.65 CC, 19.54 mm CL diameter and in primiparous as quantitative factors and when transferred to the left ipsilateral horn (9/12) with grade 1 embryos (G1: 11/ 17) as qualitative factors. However, the binary logistic regression model did not determine significance as a whole. ; El objetivo del estudio fue determinar los factores que afectan la preñez en hembras receptoras de embriones producidos in vitro bajo condiciones de altura. El estudio se llevó a cabo en la Universidad Nacional del Altiplano (UNA), ubicada en Puno, Perú, a una altitud de 3970 m. Se utilizaron 50 hembras receptoras (nulíparas, primíparas y multíparas) Brown Swiss. Se colectaron ovarios de vacas sacrificadas en un centro de beneficio local y se llevaron al laboratorio de reproducción animal de la UNA, donde se colectaron los ovocitos, y se procedió con la maduración y fertilización in vitro, cultivo y evaluación de embriones. El tiempo de transporte hasta el ...