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Protocol for structure determination of SARS-CoV-2 main protease at near-physiological-temperature by serial femtosecond crystallography

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  • معلومة اضافية
    • Contributors:
      Ertem, Fatma Betül; Güven, Ömür; Büyükdağ, Cengizhan; Göçenler, Oktay; Ayan, Esra; Yüksel, Büşra; Gül, Mehmet; Usta, Gözde; Çakılkaya, Barış; Johnson, J. Austin; Demirci, Hasan; Dağ, Çağdaş; Demirci, Hasan (ORCID 0000-0002-9135-5397 & YÖK ID 307350); Dao, E. Han; Su, Zhen; Poitevin, Frederic; Yoon, Chun Hong; Kupitz, Christopher; Hayes, Brandon; Liang, Mengning; Hunter, Mark S.; Batyuk, Alexander; Sierra, Raymond G.; Ketawala, Gihan; Botha, Sabine; Koç Üniversitesi İş Bankası Enfeksiyon Hastalıkları Uygulama ve Araştırma Merkezi (EHAM) / Koç University İşbank Center for Infectious Diseases (KU-IS CID); Graduate School of Sciences and Engineering; College of Sciences; Department of Molecular Biology and Genetics
    • بيانات النشر:
      Cell Press
      International
    • الموضوع:
      2022
    • Collection:
      Koç University Suna Kıraç Library’ Digital Collections
    • نبذة مختصرة :
      The SARS-CoV-2 main protease of (Mpro) is an important target for SARS-CoV-2 related drug repurposing and development studies. Here, we describe the steps for structural characterization of SARS-CoV-2 Mpro, starting from plasmid preparation and protein purification. We detail the steps for crystallization using the sitting drop, microbatch (under oil) approach. Finally, we cover data collection and structure determination using serial femtosecond crystallography. ; National Science Foundation (NSF) Science and Technology Centers; BioXFEL; National Institutes of Health (NIH); National Institute of General Medical Sciences (NIGMS); Scientific and Technological Research Council of Turkey (TÜBİTAK); 2232 International Fellowship for Outstanding Researchers Program of TÜBİTAK; 2244 Industrial PhD Program of TÜBİTAK; 1001 TÜBİTAK Supporting Scientific and Technological Research Projects Program; Linac Coherent Light Source (LCLS); Stanford Synchrotron Light Source (SSRL); SLAC National Accelerator Laboratory; U.S. Department of Energy, Office of Science, Basic Energy Sciences
    • File Description:
      pdf
    • ISSN:
      2666-1667
    • Relation:
      Publisher version; Koç University Institutional Repository; IR03558.pdf; Ertem, Fatma B, Omur Guven, Cengizhan Buyukdag, Oktay Gocenler, Esra Ayan, Busra Yuksel, Mehmet Gul, Gozde Usta, Baris Cakalkaya, J A. Johnson, E H. Dao, Zhen Su, Frederic Poitevin, Chun H. Yoon, Christopher Kupitz, Brandon Hayes, Mengning Liang, Mark S. Hunter, Alexander Batyuk, Raymond G. Sierra, Gihan Ketawala, Sabine Botha, Cagdas Dag, and Hasan DeMirci. ""Protocol for Structure Determination of Sars-Cov-2 Main Protease at Near-Physiological-Temperature by Serial Femtosecond Crystallography."" Star Protocols. 3.1 (2022): 101158.; https://dx.doi.org/10.1016/j.xpro.2022.101158; Scopus; PubMed; NSF-1231306; 118C270; 119C132; 120Z52; DE-AC02-76SF0051; DE-AC02-76SF00515; P41GM103393; TÜBİTAK; http://libdigitalcollections.ku.edu.tr/cdm/ref/collection/IR/id/10416
    • الرقم المعرف:
      10.1016/j.xpro.2022.101158
    • الرقم المعرف:
      edsbas.8EFC02B3