نبذة مختصرة : Cos7 cells transfected with pNLGFP, treated with interferon-alpha 24 h post transfection and then frozen 24 h post interferon treatment. Sample #C111 ; Tilt Series Date: 2012-02-18 Data Taken By: Cora Woodward Species / Specimen: Cercopithecus aethiops (monkey, African green) Strain: COS-7 ATCC CRL-1651 Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1.0°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. Acquisition Software: UCSF tomo Upload Method: rundir Processing Software Used: imod Collaborators and Roles: pNLGFP proviral construct provided by Irvin Chen UCLA. Sample prepared and imaged at Caltech by Cora Woodward Purification / Growth Conditions / Treatment: whole cells grown on grids and transfected with lipofectamine Sample Preparation: manual blotting ; Files available via S3 at https://renc.osn.xsede.org/ini210004tommorrell/tomography_archive/cwl2012-02-18-11 12feb18_C111_pNL_INF_AT1BT10012_full.rec, Reconstruction (Pixel Size 2.0 nm), 619.4 MB Download 12feb18_C111_pNL_INF_AT1BT10012.mrc, Tilt Series (Pixel Size 0.5 nm), 1.0 GB Download 12feb18_C111_pNL_INF_AT1BT10012_full.tiff, file associated to 3D Image #68747, 788.3 kB Download
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