Additional file 1 of Extracellular matrices of stromal cell subtypes regulate phenotype and contribute to the stromal microenvironment in vivo ...

Supplementary Figure 1: A Quantification of senescence associated beta-galactosidase staining at 72 hours. B Heatmap of microarray-derived data showing Log2 fold-change in expression of genes identified in the Reactome_senescence genes database in Y201 and Y202 compared to their parental cells. The classical senescence-associated genes CDKN1A, CCL2 and TP53 were not detected by the microarray and are not represented in the heatmap. C Outline of the CellProfiler pipeline for detection and quantification of crystal violet stained CFU-F assays. Supplementary Figure 2. A Quantification of focal adhesion size following fluorescent staining of Y202 cells treated with either Y201-CM or Y202-CM for 24 hours. B Mean colony area from in vitro aged primary BMSC CFU-Fs (n=5) (ANOVA: F = 3.863, df = 1.833, 7.332, p = 0.0738) C Total colonies identified from CFU-Fs of in vitro aged primary BMSCs (Friedman test, p = 0.0085) with post-hoc test revealing significant effect of Y201CM vs no CM (p = 0.0044) n = 5. *p≤0.05, ...