Figure 7—figure supplement 1. Sequence Alignment of FKF1/ZTL family members in plants. ; ZTL/FKF1/LKP2 homologs from dicots; (Arabidopsis thaliana (At), Brassica rapa (Bra), Glycine max (Gm), Cucumis sativus (Cs), Citrus clementine (Cc), Gossypium hirsutum (Gh), Avena sativa (As), monocots; Oryza sativa japonica (Os), Setaria italica (Si), Zea mays (Zm), Brachypodium distachyon (Bd), Liverwort; Marchantia polymorpha (Mp) and Spikemoss; Selaginella moellendorffii (Sm). ZTL and FKF1 cluster in reference to conserved CGF and QFF motifs. See Figure 7 for corresponding accession numbers. All ZTL proteins conserve G46 and V48 (blue). In FKF1 the position corresponding to G46 contain Ala (FKF1-like monocots) or Ser (FKF1-like dicots) (red); Phototropins contain an Asn at the equivalent position (green). * denote conserved residues through all proteins. All proteins conserve the canonical GXNCRFLQ motif (magenta) as well as residues leading into the E-F loop. The FKF1 species differ in the residues immediately following the LOV consensus sequence in the beginning of the E-F loop (ZTL: C87 and G89; FKF1: F87 and D89). Liverwort and spikemoss sequences diverge containing elements consistent with both ZTL and FKF1 (G46 and ZTL E-F loop but I48 for Mp; S46 and F87 but G89 for Sm), indicating an evolutionary transition. The QFF (blue) motif is more divergent. All ZTL/FKF1 contain a Phe at position 156 that occupies an alternative buried position compared to solvent exposed hydrophilic residues in other LOV proteins.

  Processing Request