Individual mito-bulb organelles persist over the course of more than 4 h.

A . Single image frame shows RPMI-adapted A549 cells expressing MLS-EGFP imaged for over 4 h at 5 min intervals via live, z-stack, confocal microscopy. The ROI (red box) denotes a swollen mitochondria, displayed throughout image collection at the indicated 35-min intervals. Scale bar is 15 μm. Leica SP8, 63x, 0.75x zoom, 5% CO 2 , 37⁰C, 95% relative humidity. B . Mitochondrial diameters of several individual mito-bulbs are plotted with respect to time, measured at 5 min intervals, showing stability over the course of more than 4 h. Graph represents the measure of the smaller elliptical diameter with respect to time for each mito-bulb measured in A.