Insecticidal Activity of 28 Essential Oils and a Commercial Product Containing Cinnamomum cassia Bark Essential Oil against Sitophilus zeamais Motschulsky ; 어리쌀바구미(Sitophilus zeamais)에 대한 시나몬(Cinnamomum cassia) 정유를 함유한 제품과 28종 에센셜 오일의 살충 활성

학위논문 (석사) -- 서울대학교 대학원 : 농업생명과학대학 농생명공학부, 2021. 2. 탁준형. ; The maize weevils, Sitophilus zeamais, are stored product pests mostly found in warm and humid regions around the globe. In the present study, acute toxicity via contact and residual bioassay and fumigant bioassay of 28 essential oils as well as their attraction–inhibitory activity against the adults of S. zeamais were evaluated. Chemical composition of the essential oils was analyzed by gas chromatography-mass spectrometry, and a compound elimination assay was conducted on the four most active oils (cinnamon, tea tree, ylang ylang, and marjoram oils) to identify major active constituents. Amongst the oils examined, cinnamon oil was the most active in both contact/residual and fumigant bioassays and exhibited strong behavioral inhibitory activity. Based on the compound elimination assay and chemical analyses, trans-cinnamaldehyde in cinnamon oil, and terpinen-4-ol in tea tree and marjoram oils were identified as the major active components. Although cinnamon oil seemed promising in the lab-scale bioassay without rice grains, it failed to exhibit strong insecticidal activity when the container was filled with rice. When a cinnamon oil-based product was applied both in an empty glass jar and a rice-filled container, all weevils in the empty jar were killed, whereas fewer than 15% died in the rice-filled container. Further study is required to understand the absorption of active fumigants by grains, and to enhance efficacy through the formulation for household products. ; 어리쌀바구미(Sitophilus zeamais)는 따뜻하고 습한 지역에서 발견되는 대표적인 저장곡물 해충의 하나이다. 본 연구에서는 어리쌀바구미에 대한 28종 에센셜 오일의 접촉 및 훈증 독성 그리고 유인 저해 효과를 평가하였다. 가스 크로마토그래프 질량분석법(GC-MS)을 이용해 에센셜 오일의 주요 성분들이 확인하였다. 또한 주성분 소거 평가(compound elimination assay)를 진행하여 독성 평가에서 효과가 좋았던 시나몬, 티트리, 일랑일랑, 마조람 에센셜 오일의 주요 활성 성분을 파악하였다. 평가된 에센셜 오일들 중에서 시나몬 오일이 독성평가와 유인 저해 평가에서 가장 뛰어난 효과를 보였다. GC-MS 분석과 주성분 소거 평가 결과를 토대로 시나몬 오일의 trans-cinnamaldehyde와 티트리, 마조람 오일의 terpinen-4-ol 이 독성에 관여하는 주요 성분이라는 것을 확인할 수 있었다. 시나몬 오일은 쌀을 넣지 않았던 용기 평가에서는 ...