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Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

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اقرأ أكثر حفظ في قائمتي
  • نوع التسجيلة:
    text
  • اللغة:
    English
  • معلومة اضافية
    • Contributors:
      Ishchenko, Andrii (Author); Cherezov, Vadim (Author); Liu, Wei (ASU author); College of Liberal Arts and Sciences; School of Molecular Sciences; Biodesign Institute; Applied Structural Discovery
    • الموضوع:
      2016
    • Collection:
      Arizona State University: ASU Digital Repository
    • نبذة مختصرة :
      We describe procedures for the preparation and delivery of membrane protein microcrystals in lipidic cubic phase for serial crystallography at X-ray free-electron lasers and synchrotron sources. These protocols can also be applied for incorporation and delivery of soluble protein microcrystals, leading to substantially reduced sample consumption compared to liquid injection. ; The final version of this article, as published in Journal of Visualized Experiments, can be viewed online at: https://www.jove.com/video/54463/preparation-delivery-protein-microcrystals-lipidic-cubic-phase-for
    • File Description:
      8 pages
    • ISSN:
      1940-087X
    • Relation:
      JOURNAL OF VISUALIZED EXPERIMENTS; Ishchenko, A., Cherezov, V., & Liu, W. (2016). Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography. Journal of Visualized Experiments, (115). doi:10.3791/54463; http://hdl.handle.net/2286/R.I.45316
    • الرقم المعرف:
      10.3791/54463
    • Rights:
      http://rightsstatements.org/vocab/InC/1.0/ ; http://creativecommons.org/licenses/by-nc-nd/4.0
    • الرقم المعرف:
      edsbas.86FCB0D8