Curcuma xanthorrhiza oil 및 죽염배합세치제의 치은염억제효과 ; Gingivitis suppression effect of the de novo dentifrice containing Curcuma xanthorrhiza, bamboo salt and various additives

Objectives: This study aimed to evaluate the gingivitis suppression effect of the dentifrice containing bamboo salt, Curcuma Xanthorrhiza, Dipotassium glycyrrhizinate, Ursodeoxycholic acid and various additives clinically and to estimate Matrix metalloproteinases in Etro and in Evo. Methods: 1. In vitro : PDL cells which were cultured succeeding a generation were treated by the mixture of Curcuma Xanthorrhiza, Dipotassium glycyrrhizinate and Ursodeoxycholic acid. After the treatment of porphyromonas gingivallis culture fluid(l5m/㎖), they were cultured for 3 days. Gelatin zymography quantified the amount of MMP-2. 2. In vivo clinical intervention study : This study was designed as a randomized blined clinical trial with factorial design using split mouth method and experimental gingivitis model. 3. Subjects : 57 Korean male adults participated this study by a written consent. All subjects were randomized into 3 groups. Twice-a-day tooth brushing was recommended for 5 weeks, and plaque index and gingival index were evaluated at the baseline, lst, 3rd, 5th weeks. In Toothshield model, the Toothshield was used for 3 weeks to evaluate gingival index and collect gingival crevicular fluid in the shielded sulcus. For the recovery the usual tooth-brushing was recommended for 2 weeks. Repeated measure ANCOVA was used for the time series comparison and ANCOVA for the comparison between the groups. Results: The de novo dentifrice suppressed the amount of MMP-2 dose-dependently in vitro. In toothbrushing model using the dentifrices, the de novo toothpaste reduced gingival index more 49.9% compared with placebo(p=0.024). In toothshield model, the de novo toothpaste reduced more 38.1% for gingival index and more 13.4% for MMP-9 than placebo, which were not statistically significant. Conclusion: The dentifrice containing bamboo salt, Curcuma Xanthorrhiza, Dipotassium glycyrrhizinate, Ursodeoxycholic acid and various additives could be a useful dentifrice for reducing gingivitis. ; 이 연구는 LG생활건강에서 연구비를 지원하였고 서울대학교 치과병원 ...