Triploid striped catfish Pangasianodon hypophthalmus: growth performance and gonadal development

This study was aimed to evaluate the growth performance and gonadal development of diploid and triploid striped catfish Pangasianodon hypophthalmus. Triploids were produced through a heat shock method at 42 °C for two minutes, at two minutes after fertilization. Before treatment performed, group of triploid and diploid were separated through nucleolus counting confirmed cromoseme counting. Five individual of each group at the age of nine months were tagged and reared for two months. Parameter of growth performance, feed conversion ratio, and survival rate were analysed using independent-samples t-test at confidence interval 95%, while gonadosomatic index (GSI) and gonad histology were analysed descriptively. No significant differences were observed between diploid and triploid fish in terms of growth performance, feed conversion ratio, and survival rate (P>0.05) during the two months rearing period, while GSI was higher in diploid (P<0.05) compared to triploid females. Histological observations of triploid female gonads showed early development stage, indicating sterility through symptoms such as oocytes degradation and abnormal development. Meanwhile, male gonad developed faster compared to female as spermatids were found in several lobules. As conclusion, diploid and triploid striped catfish P. hypophthalmus growth performance did not differ up to the age of 11 months, although some sterility symptoms were observed in both male and female. Keywords: diploid, triploid, growth, gonad, Pangasianodon hypophthalmus ABSTRAK Penelitian ini bertujuan untuk mengevaluasi performa pertumbuhan dan perkembangan gonad ikan patin siam Pangasianodon hypophthalmus diploid dan triploid. Triploid diproduksi dengan kejut suhu panas pada suhu 42 °C selama dua menit, pada dua menit setelah fertilisasi. Sebelum pengujian, kelompok diploid dan triploid dipisahkan dengan menganalisis jumlah nukleolus yang dikonfirmasi dengan penghitungan jumlah kromosom. Masing-masing lima ekor ikan diploid dan triploid pada umur ...