The Possible Protective Effect of bone marrw transplantation with some natural products against cytogenetical aberrations and liver cell damage induced by carbon tetrachloride

Liver fibrosis is a major health problem that can lead to liver cirrhosis and hepatic carcinoma. This study investigates the hepatoprotective effect of bone marrow mononuclear cells (BM-MNCs) transplantation, N-acetylcysteine (NAC) and ?-lipoic acid (ALA) against hepatic toxicity and cytogenetical aberrations experimentally induced by carbon tetrachloride (CCl4) in rats. Rats are administrated CCl4 (1mg/kg, i.p.) twice/week for 8 weeks showed elevation of liver enzymes ALT, AST in serum, liver tissue TNF-?, IL-6 inflammatory cytokines, while a reduction of albumin serum level and IL-10 anti-inflammatory cytokines, oxidative stress biomarkers as SOD, CAT, GPx, TAC liver tissue content accompanied by elevation of MDA liver content. In addition to cytogenetical aberration assessment via micronucleated polychromatic erythrocytes (MnPCEs) count, percent (%) of DNA damaged cells. Administration of BM-MNCs (1×106 in 0.1ml PBS, i.v.), NAC (300 mg/kg, p.o), ALA (100 mg/kg, p.o) alone and their combinations (BM-MNCs with NAC and BM-MNCs with ALA) decreased tissue content of TNF-?, IL-6, MDA, percentage of DNA damaged cells and MnPCEs and increase others as GPx, CAT, SOD, TAC and IL-10. Also, these agents produced marked reduction of liver enzymes ALT, AST, serum albumin. Notably, BM-MNCs and NAC or BM-MNCs and ALA combinations exerted significant antioxidant, anti-inflammatory and anti-cytogenetical aberrations effect compared to each of them alone. In conclusion, NAC, ALA, BM-MNCs can be probably introduced as candidates for protection of liver toxicity caused by CCl4. Keywords:hepatotoxicity; oxidative stress; hepatoprotective agent; Mutagenicity testing