نبذة مختصرة : Background: Some procedures that are performed during the daily dental practice might favor environmental contamination by microorganisms. Therefore, their presence in materials that are routinely used in the dental office constitutes a risk of transmitting pathogen agents, thereby causing cross-contamination. Aim: To evaluate microbial contamination of light-curing composites previously used in patients at the School of Dentistry, Universidad Cooperativa de Colombia, Envigado campus.Materials and Methods: The experimental group consisted of 46 composite samples extracted from previously-used composite syringes. Composite from two as-received syringes and two sterile toothpicks were used as the control group. Samples were deposited in BHI broth for 48 hours at 37°C. After this time, a turbidity reading was performed. Contaminated samples were seeded in solid culture media and colonies were described according to their macro and microscopic characteristics. The SPSS 21.0 statistical software was used and Chi-square coefficient was calculated.Results: 34.8% of composites were contaminated by Staphylococcus sp and Gram positive bacillus. No statistical significant differences between composite contamination and frequency of use were observed (p>0,05). Conclusions: Composite microbial contamination after a single use was demonstrated. Therefore, composite remnants contained in previously-used syringes might be responsible for cross-contamination. ; Background: Some procedures that areperformed during the daily dental practicemight favor environmental contaminationby microorganisms. Therefore, theirpresence in materials that are routinelyused in the dental office constitutes a riskof transmitting pathogen agents, therebycausing cross-contamination.Aim: To evaluate microbial contaminationof light-curing composites previouslyused in patients at the School of Dentistry,Universidad Cooperativa de Colombia,Envigado campus.Materials and Methods: The experimentalgroup consisted of 46 composite samplesextracted from previously-used compositesyringes. Composite from two as-receivedsyringes and two sterile toothpicks wereused as the control group. Samples weredeposited in BHI broth for 48 hours at37°C. After this time, a turbidity readingwas performed. Contaminated sampleswere seeded in solid culture media andcolonies were described according to theirmacro and microscopic characteristics. TheSPSS 21.0 statistical software was usedand Chi-square coefficient was calculated.Results: 34.8% of composites were contaminatedby Staphylococcus sp and Grampositive bacillus. No statistical significantdifferences between composite contaminationand frequency of use were observed(p>0,05). Conclusions: Composite microbial contaminationafter a single use was demonstrated.Therefore, composite remnants containedin previously-used syringes might beresponsible for cross-contamination. ; Introducción: Durante la práctica odontológica se desarrollan procedimientos que favorecen la contaminación del ambiente de trabajo, por consiguiente, la presencia de microorganismos en los materiales empleados en la atención clínica constituyen un riesgo de transmisión de agentes patógenos ocasionando infecciones cruzadas.Objetivo: Evaluar la contaminación microbiana en las resinas de fotocurado utilizadas en la atención clínica de los pacientes que asisten a las clínicas de la Facultad de Odontología de la Universidad Cooperativa de Colombia, sede Envigado.Materiales y Métodos: Se conformó un grupo experimental de 46 muestras de resina manipuladas por los estudiantes durante la atención clínica; dos jeringas de resina nuevas y dos palillos estériles conformaron el grupo control. Las muestras de resina se depositaron en caldo BHI y después de 48h de incubación a 37°C se realizó una lectura de la turbidez en los caldos. Las muestras contaminadas se sembraron en medios de cultivo sólidos y las colonias fueron descritas por sus características macroscópicas y microscópicas. Se utilizó el programa estadístico SPSS 21.0 y se calculó el coeficiente Chi-cuadrado.Resultados: El 34,8% de las resinas estaban contaminadas por bacterias del género Staphylococcus y bacilos Gram positivos. No se observaron diferencias estadísticamente significativas entre la contaminación de las resinas y la frecuencia de uso (p>0,05)Conclusiones: Se comprobó que con sólo un uso de la resina después del procedimiento clínico en un paciente es suficiente para promover su contaminación, por lo tanto el remanente de la resina puede ser considerado como uno de los vehículos responsables de contaminación cruzada.
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