Complementation of ompA , ompC , and waaC mutants.

Top agar spot assays with the 13 phages highlighted in S8 Fig were performed with the ompA (top) and ompC (middle) mutant strains of E . coli K-12 BW25113 as well as the waaC mutant of E . coli K 12 MG1655 ΔRM (bottom). The plates on the left side were generated with strains carrying the respective complementation plasmid (see Materials and methods ) and the plates on the right side with E . coli carrying the ev control. The plates show that the ompA dependency of PaulHMueller (Bas45; top) and the ompC dependency of FelixPlatter (Bas40; middle) could be fully complemented. Similarly, the loss of infectivity of DanielBernoulli (Bas08), WilhelmHis (Bas35), AlbertHofmann (Bas47), VogelGryff (Bas25), and EmilieFrey (Bas61) on the waaC mutant could be fully complemented by genetic complementation (bottom). ev, empty vector. (TIF)