PHYTOCHEMICAL ANALYSIS OF TERPENIC COMPOUNDS IN RAW MATERIALS OF SOME REPRESENTATIVES OF THE TRIBE MENTHEAE (LAMIACEAE FAMILY) ; ФІТОХІМІЧНИЙ АНАЛІЗ СПОЛУК ТЕРПЕНОВОЇ ПРИРОДИ В СИРОВИНІ ДЕЯКИХ ПРЕДСТАВНИКІВ ТРИБИ MENTHEAE (РОДИНА LAMIACEAE)

The aim of the work. Phytochemical analysis of terpenic compounds in the herbs of three representatives of the tribe Mentheae belonging to the Lamiaceae family (Dracocephalum moldavica L., Lophanthus anisatus (Nutt.) Benth and Satureja hortensis L.) by chromatographic and spectrophotometric methods. Materials and Methods. The herbs of plants were harvested from the experimental plots in Ternopil region (Ukraine) at the beginning of their full flowering. Essential oils were obtained using hydrodistillation. The thin layer chromatography (TLC) method was used to identify terpenic compounds. The component composition of triterpenoids was determined by the high performance liquid chromatography (HPLC) analysis. The total contents of triterpenoids were determined spectrophotometrically. Results and Discussion. The dominant components and specific "chromatographic fingerprints" of the essential oils as well as non-volatile terpene compounds of the herbs such as ursolic acid and β-sitosterol were determined using TLC method. The HPLC analysis found that ursolic and euscaphic acids were the main triterpenes of the herb of all studied species; euscaphic acid (0.56 %) prevailed in the raw material of Satureja hortensis, and ursolic acid (0.21 %) in Lophanthus anisatus. A decrease in the total content of triterpenoids in the direction Lophanthus anisatus (1.67±0.04) % > Satureja hortensis (1.26±0.03) % > Dracocephalum moldavica (1.05±0.03) % was determined spectrophotometrically. Conclusions. The conducted phytochemical analysis of herbs of the studied species allowed identifying their terpenic compounds and to determining the composition and total contents of triterpenoids. ; Мета роботи. Фітохімічний аналіз сполук терпенової природи у траві трьох видів триби Mentheae родини Lamiaceae (Dracocephalum moldavica L., Lophanthus anisatus (Nutt.) Benth та Satureja hortensis L.) хроматографічними та спектрофотометричним методами. Матеріали і методи. Траву заготовляли на дослідних ділянках у Тернопільській області на ...