Aggregation studies of de-metallated and reduced SOD1 WT and SOD1 A4V on treatment with PB.

Analytical SEC of metallated SOD1 WT (a), de-metallated SOD1 WT (b), metallated SOD1 A4V (c) and de-metallated SOD1 A4V (d) in the absence of PB were analyzed under reducing conditions before and after 24 hrs and 48 hrs incubation at 37ºC. The monomer (M), dimer (D), trimer (T), and large aggregate (L) species are shown as green, purple, red, and black dotted lines, respectively. All the experiments were performed for n = 3 biological replicates. (DOCX)