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A comparative transmission electron microscopy study of titanium dioxide and carbon black nanoparticles uptake in human lung epithelial and fibroblast cell lines.

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  • معلومة اضافية
    • Contributors:
      Institut Mondor de Recherche Biomédicale (IMRB); Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12); Hôpital Henri Mondor; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12); Laboratoire d'Etude des Particules Inhalées (LEPI); Ville de Paris; CHU Tenon AP-HP; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU); Laboratoire de Cytophysiologie et Toxicologie Cellulaire (LCTC); Université Paris Diderot - Paris 7 (UPD7); Service de pneumologie et pathologie professionnelle; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor; This work was supported by the "Agence Nationale de la Recherche France" (Nanotox project, ANR N° 05979-5 SET 024-01), by the Department of Paris, by the "Agence nationale de sécurité sanitaire de l'Alimentation, de l'Environnement et du Travail" for a PhD grant andfunding of Esther Belade, and by the "ABIES" PhD program for a PhD grant of Lucie Armand.
    • بيانات النشر:
      HAL CCSD
      Elsevier
    • الموضوع:
      2012
    • Collection:
      Inserm: HAL (Institut national de la santé et de la recherche médicale)
    • نبذة مختصرة :
      International audience ; Several studies suggest that the biological responses induced by manufactured nanoparticles (MNPs) may be linked to their accumulation within cells. However, MNP internalisation has not yet been sufficiently characterised. Therefore, the aim of this study was to compare the intracellular uptake of three different MNPs: two made of carbon black (CB) and one made of titanium dioxide (TiO(2)), in 16HBE bronchial epithelial cells and MRC5 fibroblasts. Transmission electron microscopy was used to evaluate the intracellular accumulation. Different parameters were analysed following a time and dose-relationship: localisation of MNPs in cells, percentage of cells having accumulated MNPs, number of aggregated MNPs in cells, and the size of MNP aggregates in cells. The results showed that MNPs were widely and rapidly accumulated in 16HBE cells and MRC5 fibroblasts. Moreover, MNPs accumulated chiefly as aggregates in cytosolic vesicles and were absent from the mitochondria or nuclei. CB and TiO(2) MNPs had similar accumulation patterns. However, TiO(2) aggregates had a higher size than CB aggregates. Intracellular MNP accumulation was dissociated from cytotoxicity. These results suggest that cellular uptake of MNPs is a common phenomenon occurring in various cell types.
    • Relation:
      info:eu-repo/semantics/altIdentifier/pmid/22036670; inserm-00673352; https://inserm.hal.science/inserm-00673352; https://inserm.hal.science/inserm-00673352/document; https://inserm.hal.science/inserm-00673352/file/2012-Belade_et_al_version_auteur_.pdf; PUBMED: 22036670
    • الرقم المعرف:
      10.1016/j.tiv.2011.10.010
    • الدخول الالكتروني :
      https://inserm.hal.science/inserm-00673352
      https://inserm.hal.science/inserm-00673352/document
      https://inserm.hal.science/inserm-00673352/file/2012-Belade_et_al_version_auteur_.pdf
      https://doi.org/10.1016/j.tiv.2011.10.010
    • Rights:
      info:eu-repo/semantics/OpenAccess
    • الرقم المعرف:
      edsbas.7247D283