نبذة مختصرة : Heterosexual HIV transmission is the most common viral transmission route worldwide. To establish a persistent infection the virus needs to cross the mucosal surface of the genital tract. The genital mucosa is thus considered to be the portal of HIV entry and initial site of viral replication. A better understanding of the immunological milieu at the portal of viral entry is crucial for the development of preventive interventions. In Paper I we investigated how herpes simplex virus 2 (HSV-2) affects the genital epithelial barrier and mucosal immune response in an HSV-2 seropositive, asymptomatic vs. HSV-2 seronegative male population in Kenya. The two study groups had comparable levels of all selected markers of inflammation and epithelial integrity, except for lower mRNA levels of the epithelial junction protein claudin-1 in the HSV-2 seropositive group, which may indicate a less robust genital epithelial barrier. In Paper II, we investigated how the use of progesterone-based hormonal contraceptives affects the genital epithelial barrier and mucosal HIV receptor expression in healthy Swedish women. The progesterone-based intrauterine device (pIUD) group was compared to a non-hormonal contraceptive (noHC) group and a combined oral hormonal contraceptives (COC) group. Similar protein expression levels of HIV receptors and co-receptors were observed in the three study groups. However, women using pIUD displayed a thinner apical layer of the ectocervical epithelium and lower mRNA levels of the epithelial junction protein ZO-1 as compared to the control groups. These results suggest that pIUD use may weaken the ectocervical epithelial barrier against invading pathogens, such as HIV. In Paper III, we further investigated how the use of hormonal contraceptives affects the production of antimicrobial peptides (AMPs) in different compartments of the female genital mucosa, including secretions and tissue. Women using COC had significantly lower mRNA levels of the AMPs BD-2 and trappin-2 in ectocervical tissue as compared ...
Relation: I. Comparable mRNA expression of inflammatory markers but lower claudin-1 mRNA levels in foreskin tissue of HSV-2 seropositive versus seronegative asymptomatic Kenyan young men. MARIA RÖHL, Annelie Tjernlund, Supriya D. Mehta, Pernilla Petersson, Robert C. Bailey and Kristina Broliden. BMJ Open. 2015 Feb 18; 5(2):e006627. ::doi::10.1136/bmjopen-2014-006627 ::pmid::25694458 ::isi::000363455400022; II. Progesterone-Based Intrauterine Device Use Is Associated with a Thinner Apical Layer of the Human Ectocervical Epithelium and a Lower ZO-1 mRNA Expression. Annelie Tjernlund, Ann Marie Carias, Sonia Andersson, Susanna Gustafsson-Sanchez, MARIA RÖHL, Pernilla Petersson, Andrea Introini, Thomas J. Hope and Kristina Broliden. Biology of Reproduction. 2015 Mar; 92(3):68. ::doi::10.1095/biolreprod.114.122887 ::pmid::25588510 ::isi::000350808500012; III. Expression profiles of antimicrobial peptides in the genital tract of women using progesterone intrauterine devices versus combined oral contraceptives. Andrea Introini, Tove Kaldensjö, Taha Hirbod, MARIA RÖHL, Annelie Tjernlund, Sonia Andersson and Kristina Broliden. American Journal of Reproductive Immunology. 2014 Nov; 72(5):475-84. ::doi::10.1111/aji.12304 ::pmid::25087845 ::isi::000343731800004; IV. Intact tissue microenvironment and HIV target cell expression in the ectocervical epithelium of HIV-exposed seronegative sex workers. MARIA RÖHL, Annelie Tjernlund, Julie Lajoie, Gabriella Edfeldt, Genevieve Boily-Larouche, Muhammad Asghar, Julianna Cheruiyot, Makubo Kimani, Joshua Kimani, Julius Oyugi, Keith R. Fowke, Kristina Broliden. [Manuscript]; http://hdl.handle.net/10616/46372
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