نبذة مختصرة : Background: Obesity in men, particularly when central, is associated with lower total testosterone [TT], free testoster-one [FT] and sex hormone-binding globulin [SHBG], and a greater decline in TT and FT with increasing age compared with lean men. Obesity-related conditions such as obstructive sleep apnea, insulin resistance and type 2 diabetes mellitus are independently associated with decreased plasma testoster-one. Obese men have reduced sperm concentration and total sperm count compared to lean men but sperm motility and morphology appear unaffected. The cause and effect relation-ships between low plasma androgen levels, obesity and the metabolic syndrome remain unclear. Adiponectin has a role in the control of the reproductive axis, which might operate as endocrine integrator linking metabolism and gonadal function. Expression, regulation and functional role of adi-ponectin and resistin adipokines in rat testis were documented. Both adipokines may operate as novel endocrine integrators linking energy homeostasis and reproduction.Objective: The aim of the present study was to find out an effect of obesity on testicular mRNA gene expression of adiponectin and/or resistin adipokines. Also, to investigate a potential correlation between the mRNA expression of adi-ponectin and/or resistin and testicular function as indicated by serum testosterone level in obese male rats.Material and Methods: The study was conducted on 20 male albino rats. The experimental animals were divided into 2 groups, 10 rats each. Group I: Control rats and Group II: Obese rats. After 12 weeks, all overnight-fasting rats were sacrificed. Blood samples were collected, and the testes were rapidly excised, weighed and frozen at –80ºc for further mRNA detection. The following parameters were measured: Serum fasting glucose, fasting insulin, fasting triglycerides, fasting cholesterol, insulin resistance was estimated using homeostatic model assessment (HOMA) index, serum test-osterone, testicular resistin mRNA expression and testicular ...
Processing Request
No Comments.