In vivo monitoring of hepatic glycolipid distribution of n-6 ∕ n-3 in jugular-vein-cannulated rats as a nutritional research model for monogastric animal

The metabolic distribution via blood from liver of glycerolipids by omega-6 to omega-3 fatty acid ( n -6  ∕   n -3) ratio in monogastric animal nutrition is very important. In vivo monitoring technique using jugular-vein-cannulated rats as a nutritional model for monogastric animal can yield important insights into animal nutrition. This study was conducted to determine the effect of different n -6  ∕   n -3 ratios ( 71:1 , 4:1 , 15:1 , 30:1 ) on metabolic distribution of glycerolipids newly synthesized and secreted in the liver of the rats and explore the mechanism involved. Regarding 14 CO 2 released from oxidation of glycerolipid metabolism, it was the highest (22.5 %) in groups with a n -6  ∕   n -3 ratio of 4:1 ( P <0.05 ). The control group showed the highest total glycerolipid level, followed by the 30:1 , 15:1 , and 4:1 groups in order ( P <0.05 ). When secreted triacylglycerol level of each group was compared with that of the control group, the 4:1 , 15:1 , and 30:1 groups were decreased by 36.3 %, 20.9 %, and 13.3 %, respectively ( P <0.05 ). Regarding the distribution of phospholipid against total glycerolipid compared to the control group, the 4:1 , 15:1 , and 30:1 groups were 1.38, 1.29, and 1.17 times higher, respectively ( P <0.05 ). In the comparison of 14 CO 2 emission against total glycerolipid compared with the control group, the 4:1 , 15:1 , and 30:1 groups were 1.61, 1.52, and 1.29 times higher, respectively ( P <0.05 ). These results demonstrate that a dietary n -6  ∕   n -3 fatty acid ratio of 4:1 could significantly decrease harmful lipid levels in the blood by controlling the mechanism of metabolic distribution via blood from triglyceride and phospholipid newly synthesized in the liver of cannulated rat.