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Characterization of mannose-binding lectin plasma levels and genetic polymorphisms in HIV-1-infected individuals ; Caracterização dos polimorfismos genéticos e dos níveis plasmáticos da lectina ligante de manose em indivíduos infectados pelo HIV-1

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  • معلومة اضافية
    • الموضوع:
      2013
    • Collection:
      Universidade Federal do Pará: Repositório Institucional da UFPA
    • نبذة مختصرة :
      INTRODUCTION: The present study investigated the association between mannose-binding lectin (MBL) gene polymorphism and serum levels with infection by HIV-1. METHODS: Blood samples (5mL) were collected from 97 HIV-1-infected individuals resident in Belém, State of Pará, Brazil, who attended the Special Outpatient Unit for Infections and Parasitic Diseases (URE-DIPE). CD4+ T-lymphocyte count and plasma viral load were quantified. A 349bp fragment of exon 1 of the MBL was amplified via PCR, using genomic DNA extracted from controls and HIV-1-infected individuals, following established protocols. MBL plasma levels of the patients were quantified using an enzyme immunoassay kit. RESULTS: Two alleles were observed: MBL*O, with a frequency of 26.3% in HIV-1-infected individuals; and the wild allele MBL*A (73.7%). Similar frequencies were observed in the control group (p > 0.05). Genotype frequencies were distributed according to the Hardy-Weinberg equilibrium in both groups. Mean MBL plasma levels varied by genotype, with statistically significant differences between the AA and AO (p < 0.0001), and AA and OO (p < 0.001) genotypes, but not AO and OO (p = 0.17). Additionally, CD4+ T-lymphocytes and plasma viral load levels did not differ significantly by genotype (p > 0.05). CONCLUSIONS: The results of this study do not support the hypothesis that MBL gene polymorphism or low plasma MBL concentrations might have a direct influence on HIV-1 infection, although a broader study involving a large number of patients is needed. ; INTRODUÇÃO: O presente estudo investigou a associação entre o polimorfismo no gene da lectina ligante de manose (MBL) e os níveis séricos da proteína com a infecção pelo HIV-1. MÉTODOS: As amostras de sangue (5mL) foram coletadas de 97 indivíduos infectados pelo HIV-1 residentes em Belém, Estado do Pará, Brasil, que frequentavam a Unidade de Referência Especial para Doenças Infecciosas e Parasitárias Especiais (URE-DIPE). Os níveis de linfócitos T CD4+ e da carga viral plasmática foram ...
    • File Description:
      application/pdf
    • ISSN:
      1678-9849
      0037-8682
    • Relation:
      VALLINOTO, Antonio Carlos Rosário, et al. Characterization of mannose-binding lectin plasma levels and genetic polymorphisms in HIV-1-infected individuals. Revista da Sociedade Brasileira de Medicina Tropical. Uberaba, v. 44, n. 1, p. 1-3, jan./fev. 2011. Disponível em: . Acesso em: 25 mar. 2013. .; http://repositorio.ufpa.br/jspui/handle/2011/3601
    • الدخول الالكتروني :
      http://repositorio.ufpa.br/jspui/handle/2011/3601
    • Rights:
      Acesso Aberto
    • الرقم المعرف:
      edsbas.6BC90B90