Item request has been placed! ×
Item request cannot be made. ×
loading  Processing Request

Non-apical mitoses contribute to cell delamination during mouse gastrulation

Item request has been placed! ×
Item request cannot be made. ×
loading   Processing Request
  • معلومة اضافية
    • Contributors:
      Unité de biologie Moléculaire, Cellulaire et du Développement (MCD); Centre de Biologie Intégrative (CBI); Université Toulouse III - Paul Sabatier (UT3); Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3); Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS); Institut de Recherche Interdisciplinaire en Biologie Humaine et moléculaire = Insitute of Interdisciplinary Research Bruxelles, Belgique (IRIBHM); Faculté de Médecine Bruxelles (ULB); Université libre de Bruxelles (ULB)-Université libre de Bruxelles (ULB); MRC, Lab Mol Biol, Cambridge, England; Partenaires INRAE; Kyoto University Institute for Advanced Study (ASHBi); Faculty of Mathematics, University of Vienna; ANR-21-CE13-0028,SingleCrest,SINGLE CREST: Mosaïque cellulaire et crête neurale: impact sur la transition épithélium-mésenchyme.(2021); European Project; European Project: PJA-20191209284
    • بيانات النشر:
      HAL CCSD
      Nature Publishing Group
    • الموضوع:
      2024
    • نبذة مختصرة :
      International audience ; During the epithelial-mesenchymal transition driving mouse embryo gastrulation, cells divide more frequently at the primitive streak, and half of those divisions happen away from the apical pole. These observations suggest that non-apical mitoses might play a role in cell delamination. We aim to uncover and challenge the molecular determinants of mitosis position in different regions of the epiblast through computational modeling and pharmacological treatments of embryos and stem cell-based epiblast spheroids. Blocking basement membrane degradation at the streak has no impact on the asymmetry in mitosis frequency and position. By contrast, disturbance of the actomyosin cytoskeleton or cell cycle dynamics elicits ectopic non-apical mitosis and shows that the streak region is characterized by local relaxation of the actomyosin cytoskeleton and less stringent regulation of cell division. These factors are essential for normal dynamics at the streak and favor cell delamination from the epiblast. Epithelial-mesenchymal transition (EMT) is a reversible process by which a static epithelial cell becomes motile through the acquisition of a mesenchymal phenotype. It involves a loss or change in intercellular junctions, reorganization of the cytoskeleton, a switch from apicalbasal to front-rear polarity, and remodeling of the extracellular matrix 1,2 . EMT is a fluid non-linear spectrum in which cells can adopt intermediate phenotypes with various levels of epithelial and mesenchymal features 2 . Gastrulation, an evolutionary conserved developmental event through which multiple germ layers arise from a single epithelium, occurs through EMT-mediated cell delamination. In the mouse embryo, gastrulation takes place in the primitive streak (PS), a structure specified in the posterior region of the epiblast at embryonic day (E) 6 3 . One of the earliest steps of gastrulation EMT is the degradation of the epiblast basement membrane 4,5 . Perforations are already present globally before the onset of ...
    • Relation:
      info:eu-repo/grantAgreement//PJA-20191209284/EU/Fondation ARC pour la recherche sur le cancer/; hal-04688863; https://cnrs.hal.science/hal-04688863
    • الرقم المعرف:
      10.1038/s41467-024-51638-6
    • Rights:
      http://creativecommons.org/licenses/by-nc/
    • الرقم المعرف:
      edsbas.6AB84F27