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Flow cytometry gating strategy.

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  • معلومة اضافية
    • الموضوع:
      2023
    • نبذة مختصرة :
      A) Lymphocytes were gated according to FSC and SSC, respectively. Live/dead dye-negative cells were identified as live cells. B) Macrophages were gated on CD11b + CD64 + cells. CD80 + CD206 - macrophages were characterized as M1 macrophages. Monocytes were characterized as CD11b int CD64 - cells. CD11b hi Ly6G hi subpopulation was identified as neutrophils, with CD63 as the neutrophil activation marker. C) Total T cells were gated on CD3 first, followed by CD4 and CD8 gating. CD44 + CD62L - T cells were considered activated T cells. CD3 - NK1.1 + cells were identified as NK cells, with CD44 as the activation marker. (TIF)
    • Relation:
      https://figshare.com/articles/figure/Flow_cytometry_gating_strategy_/24801970
    • الرقم المعرف:
      10.1371/journal.pntd.0011445.s003
    • Rights:
      CC BY 4.0
    • الرقم المعرف:
      edsbas.68F5CDB4