Avaliação da Atividade Antiulcerogênia do Extrato Etanólico Bruto e da Fase Hexânica de Hyptis suaveolens L Poi Maminaceae em Modelos Animais ; Evaluation of the anti-ulcerogenic activity of the crude ethanolic extract and Exanic Fractions of Hyptis Suaveolens (L.) Poit (Lamiaceae) Animals Models.

Aerial parts of Hyptis suaveolens (Lamiaceae), known as alfazema-brava , tapera-velha , erva-canudo , cheirosa and alfavacão , is used in traditional medicine to treat stomach ailments, inflammations and infections. The aim of this study was to evaluate the gastroprotection, wound healing and toxicity of Hyptis suaveolens, with a view to validate its efficacy, safety as well as to corroborate existing data. To this end, behavioral evaluation and acute toxicity study of the ethanol extract (EEtOH-Hs) and hexane fraction (FaHex-Hs) at a single dose of 2.000 mg/kg (p.o) was performed in mice. Pharmacological effects, in particular, the gastroprotective activities of EEtOH-Hs and FaHex-Hs were evaluated. Initial pharmacological screening was conducted with EEtOH-H, Hs-FaHex, dichloromethanic fraction (FaDCM-Hs) and ethyl acetate fraction (FaAE-Hs) against acidified ethanol-induced gastric ulcers. Acute gastric ulcers induced by ethanol, NSAIDs, immobilization and cold stress, at the were carried out at doses of 62.5, 125, 250 and 500 mg / kg (po), and at 250 mg/kg (id) in gastric juice containment by pylorus ligature and ischemia-reperfusion. To evaluate ulcer healing property of EEtOH-Hs and FaHex-Hs, acetic acid-induced ulcers experiment was realized. In addition the toxicity of EEtOH-Hs and FaHex-Hs by repeated dosing for 14 days was also assessed using acetic acid ulcer model. The antimicrobial effect against Helicobacter pylori bacterium was investigated by agar diffusion method. Gastric juice parameters were evaluated in the pylorus ligature assay. Next, was investigated participation of mucus, sulfhydryl groups and nitric oxide (NO) in the gastroprotection and determine endogenous antioxidants parameters through the levels of lipid peroxidation (LPO) and superoxide dismutase (SOD) activity in gastric mucosa following ulcers induction by ischemia-reperfusion. Oral administration of EEtOH-Hs caused hyperactivity in the animals. However, it caused no death to none of the animals. Thus it was not possible to ...