نبذة مختصرة : Objective: Needle biopsy is a common technique used to obtain cell and tissue samples for diagnostics. Currently, two biopsy methods are widely used: (i) fine-needle aspiration biopsy (FNAB) and (ii) core needle biopsy (CNB). However, these methods have limitations. Recently, we developed ultrasound-enhanced fine-needle aspiration biopsy (USeFNAB), which employs a needle that flexurally oscillates at an ultrasonic frequency of ∼32 kHz. The needle motion contributes to increased tissue collection while preserving cells and tissue constructs for pathological assessment. Previously, USeFNAB has been investigated only in ex vivo animal tissue. The present study was aimed at determining the feasibility of using USeFNAB in human epithelial and lymphoid tissue. Methods: Needle biopsy samples were acquired using FNAB, CNB and USeFNAB on ex vivo human tonsils (N = 10). The tissue yield and quality were quantified by weight measurement and blinded pathologists’ assessments. The biopsy methods were then compared. Results: The results revealed sample mass increases of, on average, 2.3- and 5.4-fold with USeFNAB compared with the state-of-the-art FNAB and CNB, respectively. The quality of tissue fragments collected by USeFNAB was equivalent to that collected by the state-of-the-art methods in terms of morphology and immunohistochemical stainings made from cell blocks as judged by pathologists. Conclusion: Our study indicates that USeFNAB is a promising method that could improve tissue yield to ensure sufficient material for ancillary histochemical and molecular studies for diagnostic pathology, thereby potentially increasing diagnostic accuracy. ; Peer reviewed
Relation: Business Finland (Grant 5607/31/2018) and the Academy of Finland (Grants 314286, 311586 and 335799) are acknowledged for financial support. We thank the Helsinki University Central Hospital (HUS) Pathology Department for their facilities and equipment. We also thank the Department of Otorhinolaryngology\u2014Head and Neck Surgery, HUS, for providing the tissue samples. The curated data and the codes are available on reasonable request to the corresponding author (Heikki J. Nieminen, heikki.j.nieminen@aalto.fi). Business Finland (grant 5607/31/2018) and Academy of Finland (grants 314286, 311586 and 335799) are acknowledged for financial support. We thank Helsinki University Central Hospital (HUS) Pathology department for their facilities and equipment. We also thank the Department of Otorhinolaryngology - Head and Neck Surgery, HUS for providing the tissue samples.; Le Bourlout , Y , Rehell , M , Kelppe , J , Rautava , J , Perra , E , Rantanen , J , Ehnholm , G , Hayward , N , Nyman , K , Pritzker , K P H , Tarkkanen , J , Atula , T , Aro , K & Nieminen , H J 2024 , ' Ultrasound-Enhanced Fine-Needle Biopsy Improves Yield in Human Epithelial and Lymphoid Tissue ' , Ultrasound in Medicine and Biology , vol. 50 , no. 8 , pp. 1247-1254 . https://doi.org/10.1016/j.ultrasmedbio.2024.04.015; ORCID: /0000-0003-4150-3141/work/162653892; ORCID: /0000-0002-6560-1128/work/162657689; http://hdl.handle.net/10138/577850; ec15ac46-b7b6-45c1-9033-1164b58beaf4; 85195102794; 001259051600001
No Comments.