نبذة مختصرة : Additional file 1: Figure S1. Characteristics and differentiation potential of human UC-MSCs. Figure S2. Properties of UC-MSCs combined with HA in vitro and in vivo. Figure S3. A diagram illustrating the four groups and the chronological order exploring the effects and mechanisms of MSC/HA transplantation in VCD-induced POI mice. Figure S4. MSC/HA transplantation protected against VCD-induced follicle loss and alleviated cell apoptosis in vivo. Figure S5. Estrous cycles and hormone profiles in VCD-induced POI mice after transplantation. Figure S6. Safety assessment in the ovaries after fertility test. Figure S7. MSC-CM alleviated ovarian cell apoptosis induced by VCD. Figure S8. MSC-CM/HA was injected into the ovaries of VCD-induced POI mice, and 1 week after transplantation western blot showed the expression of DDX4 in the ovaries of the control, VCD, and MSC-CM/HA groups. Figure S9. BDNF and G-CSF were added to the in vitro ovarian culture system to determine their protective effects against VCD-induced damage. Figure S10. Safety assessment in the ovaries of aged mice after stem cell transplantation. Figure S11. HGF mediated the function of UC-MSCs in aged mice. MSC-CM, MSC-CM with HGF antibody, and HGF were injected into the aged mouse ovaries, and the ovaries in each group were collected 4 days or 8 weeks after injection and subjected to western blot analysis.
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