Contributors: University of Naples Federico II = Università degli studi di Napoli Federico II; Leiden University Medical Center (LUMC); Universiteit Leiden = Leiden University; Center for Proteomics and Metabolomics Leiden (CPM); Universiteit Leiden = Leiden University -Universiteit Leiden = Leiden University; Center for colloid and surface science (CSGI); Forschungszentrum Borstel - Research Center Borstel; B CUBE - Center for Molecular Bioengineering TU Dresden, Germany; Center for Molecular and Cellular Bioengineering TU Dresden, Germany (CMCB); Technische Universität Dresden = Dresden University of Technology (TU Dresden)-Technische Universität Dresden = Dresden University of Technology (TU Dresden); Plant Health Institute of Montpellier (UMR PHIM); Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM); Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM); A.S. was supported by PRIN 2017 (2017XZ2ZBK, 2019-2023); R.M. was supported by the European Research Council (ERC) under the European Union?s Horizon 2020 research and innovation program under grant agreement No 851356; F.D.L. was supported by the ERC under the Horizon Europe program under grant agreement No. 101039841 (DEBUG-GING LPS); and J.S. was supported by a German Federal Ministry of Education and Research BMBF grant (project 03Z22EN12) and a VW Foundation 'Life' grant (project 93090) . European Union (FSE, PON Ricerca e Innovazione 2014-2020, Azione I.1 "Dottorati Innovativi con caratter-izzazione Industriale") funded the Ph.D. grant to A.V. A.M. acknowledges funding by Progetto CN 2-Spoke 2 Task 2.2.2-Plant multitrophic interactions underlying agroecosys-tem stability and resilience. This work benefited from access (ITA001) to CERM/CIRMMP, Italy Centre of Instruct-ERIC, a Landmark ESFRI project.; European Project: ERC 851356
نبذة مختصرة : International audience ; The ability of Methylobacterium extorquens to grow on methanol as the sole carbon and energy source has been the object of intense research activity. Unquestionably, the bacterial cell envelope serves as a defensive barrier against such an environmental stressor, with a decisive role played by the membrane lipidome, which is crucial for stress resistance. However, the chemistry and the function of the main constituent of the M. extorquens outer membrane, the lipopolysaccharide (LPS), is still undefined. Here, we show that M. extorquens produces a rough-type LPS with an uncommon, non-phosphorylated, and extensively O-methylated core oligosaccharide, densely substituted with negatively charged residues in the inner region, including novel monosaccharide derivatives such as O-methylated Kdo/Ko units. Lipid A is composed of a non-phosphorylated trisaccharide backbone with a distinctive, low acylation pattern; indeed, the sugar skeleton was decorated with three acyl moieties and a secondary very long chain fatty acid, in turn substituted by a 3-O-acetyl-butyrate residue. Spectroscopic, conformational, and biophysical analyses on M. extorquens LPS highlighted how structural and tridimensional features impact the molecular organization of the outer membrane. Furthermore, these chemical features also impacted and improved membrane resistance in the presence of methanol, thus regulating membrane ordering and dynamics.
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