Phosphate chelation over calcium impacts yeast growth and lipid production from short-chain fatty acids-rich media

Producción Científica ; Some oleaginous yeasts have the ability to produce microbial oils from alternative carbon sources, such as short-chain fatty acids (SCFAs). Nevertheless, there is still a lack of information about the possible effects that media nutrients have on yeast metabolisms when using SCFAs. For instance, inorganic phosphate (PO43-) has been reported to promote yeast growth in literature but its chelating effect over other elements such as calcium (Ca2+) is often not considered in fermentation processes while limitation of nitrogen is probably the most studied. Attending at the need to better understand the role of PO43-, this work assessed the lipid production capacity of Yarrowia lipolytica ACA DC 50109, both in synthetic and real SCFAs-rich media, at different SCFAs concentrations and PO43-:Ca2+ ratios. Reducing PO43-:Ca2+ ratio was identified to be an important factor to improve yeast growth, reaching the highest lipid content (52.7 ± 0.9 % w/w) and lipid yield (0.31 ± 0.01 w/w) in media without PO43-. These results demonstrated the importance of Ca2+ availability in the medium and nutrients interactions in yeast growth that are often underestimated. ; Ministerio de Ciencia e Innovación (PID2020-119403RBC21, PCI2021-121936, PRE2018-086477, RYC2019-027773-I)