Phytochemical Screening, Physico Chemical Properties and Evaluation of Invitro Anti-Inflammatory and Anti-Oxidant Activity on the Aqueous and Ethanolic Extract of Leaves of Hygrophila Balsamica

This research was made the first time for phytochemical identification, evaluation of some pharmacological activities, leaves of Hygrophila balsamica in-order to ascertain their traditional claims. The pharmacognostical study of leaves of powder of Hygrophila balsamica have been subjected to determination of organoleptic characteristics, physicochemical constants. These studies used to find out the quantity of soluble phytoconstituents on the given quantity of plant material, used to judge the authenticity of the plants as well as to distinguish the plant material from the adulterants or allied species. The preliminary phytochemical study of aqueous and ethanolic extract of Hygrophila balsamica showed the presence of alkaloids, glycosides, flavonoids, steroids, tannin, carbohydrate, and protein and the absence of gums and mucilage, phenols, sterols, and terpenoids. Qualitative chromatographic analysis of extracts using thin layer chromatography was performed to separate and identify the single or mixture of phytoconstituents in the extract and for the identification of different components in the extract qualitatively. Both Aqueous and Ethanol extract of leaves of Hygrophila balsamica showed the In-vitro antioxidant, anti-inflammatory, activities. Hence, the leaf can be recommended therapeutically for the investigated medicinal claims.These observations will stimulate further research in the field of phytochemistry and also in the clinical application of phytoconstituents of Hygrophila balsamica in future.