Expression and modulation of certain Nod-like receptors and their co-acting partners in macrophages and corneal epithelial cells ; Egyes Nod-like receptorok és kölcsönható partnereik expressziója és modulációja makrofágokban és korneális epitél sejtekben

In our work we described for the effect of RWE on the LPS-induced IL-1β secretion of macrophages. We demonstrated that RWE (in the presence of NADPH) has no significant effect on the secretion of IL-1β, neither in the primary macrophages, dendritic cells nor on the macrophage cell line THP-1 cells, but it has robust increasing effect on the LPS-induced IL-1β production in both of these cells. We have shown that whereas RWE alone (but not NADPH) induced some ROS production, their combined effect yielded a continuously increasing ROS level. Using ROS inhibitors, we have shown that the majority of the ROS involved in the RWE-dependent enhancement of LPS-induced IL-1β production is generated by pollen-derived NADPH oxidases. We have also shown that the increased production of IL-1β is NLRP3 inflammasome-mediated and caspase-1-dependent. We reported that RWE augments the LPS-induced gene expression of pro- and cleaved IL-1β, and also the key components of NLRP3 inflammasome. We have also shown the increased protein expression of pro- and cleaved caspase-1 and IL-1β. We have also reported that RWE induces the phosphorylation of p38 MAPK, JNK, furthermore, the phosphorylation appears to be NADPH-dependent. RWE in the presence of NADPH substantially enhances the LPS-induced p38 MAPK and JNK phosphorylation. We have also demonstrated that RWE induces the phosphorylation of AP-1 complex members c-Jun and c-Fos, which are also important participants of signaling pathways involved in IL-1β expression. The observed cooperation of RWE and LPS suggests that bacterial endotoxin contamination has an important role in the ragweed pollen-induced allergic reactions. In our second study we investigated the transcription pattern of Nod-like receptors and inflammasome components in primary human corneal epithelial cells and in HCE-T cell line. The transcription of NOD1, NOD2, NLRX1 and NLRP1 is similar in the primary cells and in the cell line. We found that the expression of NLRP3 and NLRP10 is higher in HCE-T cells, while ASC and ...