نبذة مختصرة : Outer membrane vesicles are nano-sized microvesicles shed from the outer membrane of Gram-negative bacteria and play important roles in immune priming and disease pathogenesis. However, our current mechanistic understanding of vesicle-host cell interactions is limited by a lack of methods to study the rapid kinetics of vesicle entry and cargo delivery to host cells. Here, we describe a highly sensitive method to study the kinetics of vesicle entry into host cells in real-time using a genetically encoded, vesicle-targeted probe. We found that the route of vesicular uptake, and thus entry kinetics and efficiency, are shaped by bacterial cell wall composition. The presence of lipopolysaccharide O antigen enables vesicles to bypass clathrin-mediated endocytosis, which enhances both their entry rate and efficiency into host cells. Collectively, our findings highlight the composition of the bacterial cell wall as a major determinant of secretion-independent delivery of virulence factors during Gram-negative infections.
Relation: https://publications.aston.ac.uk/id/eprint/43462/1/file.pdf; O'Donoghue, Eloise J, Sirisaengtaksin, Natalie, Browning, Douglas F , Bielska, Ewa, Hadis, Mohammed, Fernandez-Trillo, Francisco, Alderwick, Luke, Jabbari, Sara and Krachler, Anne Marie (2017). Lipopolysaccharide structure impacts the entry kinetics of bacterial outer membrane vesicles into host cells. Plos Pathogens, 13 (11)
No Comments.