Evaluation of new optical spectroscopic techniques for tissue studies

Imaging techniques have developed very fast and extensively in the past few years, becoming essential for the study of structures, properties and processes in all the fields in science. Non-invasive tissular studies have become especially popular nowadays because they permit to study processes in vivo, without the alteration of the sample under focus, which were not possible to be studied with the imaging techniques in the past. Fluorescence spectroscopy is one of the imaging techniques that has been developed towards the non-invasive studies of polymers, inorganic materials, cells and tissues; with the purpose of characterize them. The main objectives of this work have been proposed in order to further investigate about the possibilities of fluorescence spectroscopy and consisted on the evaluation of two different applications. Although the tittle of the offer is maintained, the focus of the work has changed to the evaluation of the problems that are introduced right after this lines. Both are proposed to be studied at the same time since it is possible to carry out both of them under the same system and with similar methodologies. ; Ingeniería Biomédica