نبذة مختصرة : International audience ; Intensive agricultural practices generate a considerable volume of plant protection products, the impacts of which on ecosystems and biodiversity are still not fully comprehended. These substances predominantly accumulate in aquatic environments, posing potential threats to aquatic life, particularly fish. Among these pollutants, succinate dehydrogenase inhibitors (SDHIs) have risen to prominence as fungicides effective against diverse plant pathogens. SDHIs target the enzyme succinate dehydrogenase (SDH), a key component (Complex II) of the Mitochondrial Respiratory Chain (MRC). This enzyme is conserved across species, indicating that the action of SDHIs could extend to non-target organisms, such as fish. Therefore, disruption of MRC could lead to significant metabolic changes in these organisms. This study investigates the impact of fluopyram, a SDHI fungicide, on the metabolism of goldfish (Carassius auratus), a model organism in aquatic ecotoxicology.The fish were exposed to two concentrations of fluopyram (1 and 10 μg/L) over a 21-day period, with tissue sampling occurring on days 4 and 21 of exposure. Control groups were simultaneously maintained without any exposure. Lyophilized liver samples were extracted according to a double extraction procedure, segregating the lipophilic fraction for lipidomic analysis and the hydrophilic fraction for investigation of central metabolites. IC-MS and GC-FID analyses allowed the detection and quantification of central metabolites, conventional fatty acids, and neutral lipids.The preliminary results highlight a sex-specific response to fluopyram exposure. Divergent patterns were detected in the metabolomic and lipidomic profiles of male and female goldfish, suggesting different physiological reactions to the SDHI. The mechanisms underlying these sex-specific responses and their potential implications for the fitness and survival of the species warrant further investigation.
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