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In Vitro Analysis of the Co-Assembly of Type-I and Type-III Collagen

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  • معلومة اضافية
    • Contributors:
      Selçuk Üniversitesi
    • بيانات النشر:
      SPRINGER
    • الموضوع:
      2017
    • Collection:
      Selçuk Üniversitesi: SÜ Sosyal Bilimler Enstitüsü - Dergisi Açık Arşivi
    • نبذة مختصرة :
      WOS: 000394178200005 ; PubMed: 31719849 ; An important step towards achieving functional diversity of biomimetic surfaces is to better understand the co-assembly of the extracellular matrix components. For this, we study type-I and type-III collagen, the two major collagen types in the extracellular matrix. By using atomic force microscopy, custom image analysis, and kinetic modeling, we study their homotypic and heterotypic assembly. We find that the growth rate and thickness of heterotypic fibrils decrease as the fraction of type-III collagen increases, but the fibril nucleation rate is maximal at an intermediate fraction of type-III. This is because the more hydrophobic type-I collagen nucleates fast and grows in both longitudinal and lateral directions, whereas more hydrophilic type-III limits lateral growth of fibrils, driving more monomers to nucleate additional fibrils. This demonstrates that subtle differences in physico-chemical properties of similar molecules can be used to fine-tune their assembly behavior. ; Turkish Ministry of Education fellowship ; We acknowledge using the Texas A & M Materials Characterization Facility for part of the experiments. Esma Eryilmaz was supported by the Turkish Ministry of Education fellowship.
    • ISSN:
      1865-5025
      1865-5033
    • Relation:
      CELLULAR AND MOLECULAR BIOENGINEERING; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://dx.doi.org/10.1007/s12195-016-0466-3; https://hdl.handle.net/20.500.12395/35196; 10; 41; 53
    • الرقم المعرف:
      10.1007/s12195-016-0466-3
    • الدخول الالكتروني :
      https://hdl.handle.net/20.500.12395/35196
      https://doi.org/10.1007/s12195-016-0466-3
    • Rights:
      info:eu-repo/semantics/openAccess
    • الرقم المعرف:
      edsbas.516799BC