نبذة مختصرة : Health care-associated infection (HCAI) is defined as an infection occurring in a patient in a hospital or other health care facility in whom the infection was not present or incubating at the time of admission. This incident result in excess length of stay, mortality and healthcare costs. World Health Organization (WHO) has been campaigning the patient safety program, one of it is to lower the risk of HCAI. Wash hands to be one of the effective measures to break the chain of transmission of infection. Doctors, nurses and other hospital personnel must work together to ensure that hand washing before and after each contact with the patient, whether the patient known to be infected or not. The aim of this research is to find out the relationship between knowledge and health workers’ hand washing behaviour in the department of child health at Prof dr RD Kandou Hospital Manado. This research was conducted using field survey design with observational analytic studies, in this case done by observation and questionnaires. The samples were 78. Conclusion: The result showed that from the 78 health workers there are 55 people who have poor knowledge about hand washing. As for health care workers with hand-washing behaviors are most numerous in the bad category amounted to 46 people. The results of the bivariate analysis showed the value of p = 0.160. From the result it can be concluded that there is no significant relationship between knowledge and behavior of hand washing in healthcare workers in the department of child health at Prof Dr RD Kandou Hospital Manado. Keywords: Knowledge, Behavior, Hand Washing, Hand Hygiene.   Abstrak: Health care-associated infection (HCAI) didefinisikan sebagai infeksi yang terjadi pada pasien dalam rumah sakit atau fasilitas kesehatan lainnya dimana pada awalnya infeksi tersebut tidak hadir dan tanpa bukti inkubasi pada saat masuk rumah sakit. Kejadian ini menyebabkan lenght of stay (LOS), mortalitas, dan healthcare cost meningkat. World Health Organization (WHO) telah ...
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