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Global Phylogenomics of Multidrug-Resistant Salmonella Enterica Serotype Kentucky ST198

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  • معلومة اضافية
    • Contributors:
      University of Melbourne; Monash University Melbourne; Centre National de Référence - National Reference Center Escherichia coli, Shigella et Salmonella (CNR-ESS); Institut Pasteur Paris (IP); Infectiologie et Santé Publique (UMR ISP); Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT); Laboratoire de sécurité des aliments de Maisons-Alfort (LSAl); Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES); Laboratoire de Fougères - ANSES; Danmarks Tekniske Universitet = Technical University of Denmark (DTU); Universität Hohenheim = University of Hohenheim; University of Maryland School of Medicine; University of Maryland System; Sciensano Bruxelles; Pasteur Network (Réseau International des Instituts Pasteur); London School of Hygiene and Tropical Medicine (LSHTM); The French National Reference Centre for Escherichia coli, Shigella, and Salmonella is funded by the Institut Pasteur and Santé publique France. The ‘Unité des Bactéries Pathogènes Entériques’ belongs to the ‘Integrative Biology of Emerging Infectious Diseases’ Laboratory of Excellence funded by the French Government ‘Investissement d'Avenir’ programme (grant no. ANR-10-LABX-62-IBEID). C. G. was supported by a grant from Assistance publique-Hôpitaux de Paris. K. E. H. is supported by a Senior Medical Research Fellowship from the Viertel Foundation of Australia; and the Bill and Melinda Gates Foundation, Seattle, USA.; ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010)
    • بيانات النشر:
      CCSD
      Society for General Microbiology
    • الموضوع:
      2019
    • Collection:
      Institut National de la Recherche Agronomique: ProdINRA
    • نبذة مختصرة :
      International audience ; Salmonella enterica serotype Kentucky can be a common causative agent of salmonellosis, usually associated with consumption of contaminated poultry. Antimicrobial resistance (AMR) to multiple drugs, including ciprofloxacin, is an emerging problem within this serotype. We used whole-genome sequencing (WGS) to investigate the phylogenetic structure and AMR content of 121 S. enterica serotype Kentucky sequence type 198 isolates from five continents. Population structure was inferred using phylogenomic analysis and whole genomes were compared to investigate changes in gene content, with a focus on acquired AMR genes. Our analysis showed that multidrug-resistant (MDR) S. enterica serotype Kentucky isolates belonged to a single lineage, which we estimate emerged circa 1989 following the acquisition of the AMR-associated Salmonella genomic island (SGI) 1 (variant SGI1-K) conferring resistance to ampicillin, streptomycin, gentamicin, sulfamethoxazole and tetracycline. Phylo-geographical analysis indicates this clone emerged in Egypt before disseminating into Northern, Southern and Western Africa, then to the Middle East, Asia and the European Union. The MDR clone has since accumulated various substitution mutations in the quinolone-resistance-determining regions (QRDRs) of DNA gyrase (gyrA) and DNA topoisomerase IV (parC), such that most strains carry three QRDR mutations which together confer resistance to ciprofloxacin. The majority of AMR genes in the S. enterica serotype Kentucky genomes were carried either on plasmids or SGI structures. Remarkably, each genome of the MDR clone carried a different SGI1-K derivative structure; this variation could be attributed to IS26-mediated insertions and deletions , which appear to have hampered previous attempts to trace the clone's evolution using sub-WGS resolution approaches. Several different AMR plasmids were also identified, encoding resistance to chloramphenicol, third-generation cephalosporins, carbapenems and/or azithromycin. These results ...
    • Relation:
      info:eu-repo/semantics/altIdentifier/pmid/31107206; PRODINRA: 471689; PUBMED: 31107206; PUBMEDCENTRAL: PMC6700661; WOS: 000478069000001
    • الرقم المعرف:
      10.1099/mgen.0.000269
    • الدخول الالكتروني :
      https://pasteur.hal.science/pasteur-02479758
      https://pasteur.hal.science/pasteur-02479758v1/document
      https://pasteur.hal.science/pasteur-02479758v1/file/mgen000269.pdf
      https://doi.org/10.1099/mgen.0.000269
    • Rights:
      http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess
    • الرقم المعرف:
      edsbas.4F80FD9D