Quantitative results of the analysis of human hand tissues corresponding to the work 'A gene expression and a histostructural analysis of the palmar fascia of patients affected by Dupuytren's disease'

This dataset corresponds to the quantitative data generated in the work entitled "A gene expression and a histostructural analysis of the palmar fascia of patients affected by Dupuytren’s disease". Dupuytren’s disease (DD) is a condition affecting the palmar fascia that may reduce the mobility of several fingers, thus affecting the quality of life of patients. Despite its clinical relevance, the genetic and structural mechanisms associated with this disease are still not well understood. In the present work, we have carried out a genome-wide gene expression analysis using next generation sequencing (NGS) followed by a histological, histochemical and immunohistochemical analysis of some major components of the palmar fascia in 26 DD patients and 17 control subjects without the disease (CTR). Results found 237 genes or sequences differentially expressed between DD and CTR, with those genes corresponding to several gene pathways and functions related to contractility, development and morphogenesis, differentiation, extracellular matrix (ECM), migration and ossification. In turn, the histological analysis confirmed that DD tissues showed a disorganized ECM, with non-aligned fibers, and abundant cells were found scattered along the whole tissue. CTR showed significantly higher amounts of proteoglycans revealed by alcian blue, along with versican, keratan-sulfate, myoglobin, tropomyosin 3, filamin C and titin, whereas DD showed significantly enriched in collagen fibers, especially collagen type-I, MMP-14, S-100, tubulin-beta, SMA and tenascin C, with disorganization of the elastic fibers. In general, these results confirm that a significant alteration of the tissue organization, extracellular matrix and structure is related to DD. These results could contribute to the future development of diagnostic and treatment strategies for this disease. ; Dataset contains the quantitative results of the gene expression NGS analysis and the histological evaluation of the following human tissues: Patients affected by Dupuytren’s ...