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Identification of phosphate solubilizing bacteria in a Andisol of Colombian coffee region ; Identificación de bacterias solubilizadoras de fosfatos en un Andisol de la región cafetera colombiana

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  • معلومة اضافية
    • بيانات النشر:
      Universidad Nacional de Colombia - Sede Bogotá - Instituto de Biotecnología
    • الموضوع:
      2017
    • Collection:
      Universidad Nacional de Colombia: Portal de Revistas UN
    • نبذة مختصرة :
      Phosphorus is an essential nutrient for coffee growing, however, in Colombia most of the soils have low concentrations of this element. A strategy to supply the demand is the use of phosphate solubilizing microorganisms (PSM), in that order, 26 rhizospheric bacteria of Typic melanudans soil of Cajibío (Cauca, Colombia) were isolated, in three agroecosystems: coffee at full sun exposure, coffee with shade and relic secondary forest, evaluating the phosphate solubilizing efficiency (PSE) for Ca-P, Al-P, Fe-P in the solid and liquid Pikovskaya media. It showed the following solubilization sequence: Ca-P> Al-P> Fe-P. Two isolated bacteria were identified by DNA extraction and analysis of the 16S rRNA gene as Kocuria sp, and Bacillus subtilis. Later, using HPLC were identified organic acids present in the three phosphorus sources as: citric, gluconic, D- and L-malic, D- and L-lactic acid with higher presence in Ca-P-Kocuria sp. Strong acidification was observed for Fe-P and Al-P in all conditions. ; El fósforo es un elemento esencial para el cultivo de café, sin embargo la mayoría de los suelos en Colombia presentan niveles bajos de este nutriente. La presencia de microorganismos solubilizadores de fosfatos (MSF) es una de las estrategias para suplir su demanda, en ese sentido se aislaron 26 bacterias rizosféricas de Typic melanudans de Cajibío (Cauca, Colombia), en tres agroecosistemas: café sin sombra, café con sombra y relicto de bosque secundario, evaluándoles la eficiencia solubilizadora de P (ESF) en Ca-P, Al-P, Fe-P en medios Pikovskaya sólido y líquido, obteniendo la secuencia de solubilización Ca-P > Al-P > Fe-P. Los dos aislamientos bacterianos con mayor ESF se identificaron por extracción del ADN y análisis del gen 16S rRNA como Kocuria sp, y Bacillus subtilis. Posteriormente se cuantificaron e identificaron los ácidos orgánicos presentes en las tres fuentes de fosforo por HPLC, siendo ellos los ácidos cítrico, glucónico, D- y L-málico, D- y L-láctico con mayor presencia en Ca-P- Kocuria sp. En ...
    • File Description:
      application/vnd.openxmlformats-officedocument.wordprocessingml.document; application/pdf
    • Relation:
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    • الدخول الالكتروني :
      https://revistas.unal.edu.co/index.php/biotecnologia/article/view/65966
    • Rights:
      Derechos de autor 2017 Revista Colombiana de Biotecnología ; https://creativecommons.org/licenses/by/4.0
    • الرقم المعرف:
      edsbas.4B3AF66B